INITIAL LESION OF ADULT PERIODONTITIS

该提案的主要目标是评估微生物风险因素 最初的牙周损伤 具体目标将决定 牙周病变的假定微生物风险标志物是否可以 也是初期病变的危险因素。 此外， 白细胞介素-1（IL-1）基因型改变， 龈沟液中IL-1a和IL-1b的表达，以及 将确定牙周炎的患病率和进展。 然后可以使用初始病变的可靠风险因素来识别 易感个体在疾病之前或早期阶段。 在组织破坏发生之前进行预防性干预， 比治疗患病部位更好，更便宜。 此外，委员会还认为， 如果牙周病原菌和发病机制的初步 病变可以定义，其他研究和治疗研究可以 针对适当的物种和介质的组织破坏。 将使用以下方法检查300名受试者中的假定病原体和细胞因子： 用于物种快速检测的新分子生物学技术， 包括新的螺旋体分类群，细胞因子的检测和改变 细胞因子产生的基因型。 目标1检查了 受试者具有牙周损失的微生物风险标志物或改变的 IL-1基因型，在一项横断面研究中， 牙周健康的最小牙周附着丧失（例） 受试者（对照）。 要检验的假设是，风险（或 怀疑）牙周附着丧失的标记物将更高， 与健康对照组相比。 目的2是一项前瞻性队列研究 监测从第一目标中选择的200名受试者，以确定 无论受试者是否具有附着丧失的微生物风险标志物， 改变的IL-1基因型显示比对照组更广泛的附着丧失。 没有这些风险标志物的人。 受试者将接受 全面的临床和微生物监测与测量 每隔6个月重复一次，持续两年。 临床特点 包括不同的依恋模式， 损失将被定义。 第三个目标是评估特定地点的 初始牙周病变和可培养的 龈下微生物群，培养和未培养的螺旋体分类群， 以及细胞因子IL-1a和IL-1b。 微生物学、遗传学和临床 该提案中的信息将显着促进理解 的微生物病原学的初始牙周炎，这将有 对牙周基础研究具有重要意义。

The broad objective of this proposal is to assess microbial risk factors for the initial periodontal lesion. The specific aims will determine whether putative microbial risk markers for periodontal lesions could also be risk factors for initial lesions. Also, the association between the role of an altered interleukin-1 (IL-1) genotype, with increased expression of IL-1a and IL-1b in gingival crevicular fluid, and prevalence and progression of periodontitis will be determined. Reliable risk factors for initial lesions can then be used to identify susceptible individuals either before or in early phases of disease. Preventive intervention before tissue destruction occurs would be better, and less expensive, than treating diseased sites. Furthermore, if periodontal pathogens and mechanisms of pathogenesis of initial lesions could be defined, other research and treatment studies can be targeted to the appropriate species and mediators of tissue destruction. Putative pathogens and cytokines in 300 subjects will be examined using new molecular biology technologies for rapid assays of species, including new spirochete taxa, detection of cytokines and altered genotype for cytokine production. Aim 1 examines the prevalence of subjects with microbial risk markers for periodontal loss or an altered IL-1 genotype, in a cross-sectional study comparing subjects with minimal periodontal attachment loss (cases) with periodontally healthy subjects (controls). The hypothesis to be tested is that the risk (or suspected) marker for periodontal attachment loss will be higher in cases than in healthy controls. Aim 2 is a prospective cohort study monitoring 200 subjects selected from the first aim, to determine whether subjects with microbial risk markers for attachment loss or an altered IL-1 genotype demonstrate more extensive attachment loss than subjects without these risk markers. Subjects will undergo comprehensive clinical and microbial monitoring with measurements repeated at 6-month intervals for two years. Clinical characteristics of initial lesion subjects, including different patterns of attachment loss will be defined. The third aim will evaluate site-specific associations of initial periodontal lesions and the cultivable subgingival microbiota, cultivated and uncultivated taxa of spirochetes, and cytokines IL-1a and IL-1b. Microbiologic, genetic and clinical information from this proposal will significantly advance understanding of the microbial etiology of initial periodontitis, which will have profound significance for basic studies in periodontal research.