IN VIVO IMAGING IN AUTOIMMUNE UVEITIS

急性前葡萄膜炎（AAU）是一种眼部自身免疫性疾病，是人类最常见的眼内炎症。实验性自身免疫性前葡萄膜炎（EAAU）是一种啮齿动物的前葡萄膜炎模型。它是由Lewis大鼠对来源于牛虹膜和睫状体的黑色素相关抗原（MAA）致敏产生的，由CD4+ T细胞介导。由于依赖体外组织培养系统、组织的组织学分析或来自牺牲动物的细胞/因子分析，免疫细胞在体内的行为和功能受到阻碍。体内成像技术的最新进展使小型动物模型应用的高分辨率和信号灵敏度正电子发射断层扫描（PET）仪器得以发展。我们建议将我们放射科在PET扫描和正电子发射核苷酸生产方面的专业知识与我们眼科和视觉科学科免疫学方面的专业知识结合起来，研究EAAU的免疫发病机制。本建议的具体目的是：1。相关药物工具的设计、合成和评价。2. 放射性核素标记抗体对免疫细胞功能及抗体与靶抗原结合的影响。3. 免疫细胞在EAAU中的迁移和定位。

Acute anterior uveitis (AAU), the most common form of intraocular inflammation in man, is an autoimmune disease of the eye. Experimental autoimmune anterior uveitis (EAAU) is a rodent model of AAU. It is produced in the Lewis rat by sensitization to melanin associated antigen (MAA) derived from bovine iris and ciliary body and is mediated by CD4+ T cells. The behavior and function of immune cells in vivo has been hampered by reliance on in vitro tissue culture systems, histologic analysis of tissues or the analysis of cell/factors from sacrificed animals. Recent advances in in vivo imaging techniques has allowed the development of scaled down positron emission tomography (PET) instrumentation with high-resolution and signal sensitivity for application in small animal model. We propose to combine our expertise in PET scanning and the production of positron emitting nucleotides in the Department of Radiology with our expertise in immunology in the Department of Ophthalmology and Visual Sciences to study the immunopathogenesis of EAAU. The specific aims of this proposal are: 1. Design, synthesis and evaluation of relevant pharmaceutical tools. 2. Effect of radionuclide labeled antibodies on immune cell function and binding of the antibodies to their target antigens. 3. Immune cell migration and localization in EAAU.