CELL FREE APOPTOSIS--FACTORS AND MECHANISMS

描述(改编自调查员摘要)：调查员有 开发了一种基于青蛙卵提取物的新型无细胞系统， 非洲爪哇。这种鸡蛋提取物能够复制许多 细胞凋亡的特征，包括染色质凝集、DNA内切 核子的分裂、收缩和碎裂。裂解物 杆状病毒感染细胞表达癌基因bcl2的抑制作用 核碎裂。这个系统提供了一个很好的手段来剖析 细胞凋亡的分子机制及bcl2的保护作用。这个 申请人建议鉴定和描述一些细胞质 细胞凋亡事件。这种无电池系统提供了一个机会 从分离的组分中重建细胞的凋亡过程。

DESCRIPTION (adapted from investigator's abstract): The investigator has developed a novel cell -free system based on extracts from eggs of the frog, Xenopus laevis. Such egg extracts are able to reproduce many of the features of apoptosis, including chromatin condensation, endonucleolytic DNA cleavage and the shrinkage and fragmentation of the nuclei. Lysates from baculovirus-infected cells expression the oncogene bcl-2 can suppress nuclear fragmentation. This system provides an excellent means to dissect the molecular mechanisms of apoptosis and protective function of bcl-2. The applicant proposes to identify and characterize some of the cytoplasmic events of apoptosis. Such cell-free systems offer the opportunity to reconstitute the apoptotic process from fractionated components.