BIOCHEMICAL STUDIES ON MHCK A RELATED KINASES

描述：拟议研究的长期目标是了解 蛋白激酶的生化特性和细胞调节， 控制网骨藻中肌球蛋白的定位。 建议的目标 研究的目的是描述基本的生物化学性质和结构 MHCK-A和相关的MHCK B。提出了四个具体目标：（1） 确定MHCK A的哪些残基和区域参与催化 功能和底物结合;（2）确定生物化学和 MHCK B的生理底物特异性;（3）确定是否存在 保守的自抑制或激活基序在不同的成员， MHCK激酶家族;（4）确定WD重复序列的作用 MHCK A.

DESCRIPTION: The long-term goal of the proposed studies is to understand the biochemical properties and cellular regulation of protein kinases that control myosin localization in Dictyostelium. The goal of the proposed studies is to describe the fundamental biochemical properties and structure of MHCK-A and the related MHCK B. Four specific aims are proposed: (1) To determine which residues and regions of MHCK A are involved in catalytic function and substrate binding; (2) to determine the biochemical and physiological substrate specificity of MHCK B; (3) to determine if there are conserved autoinhibitory or activating motifs in different members of the MHCK family of kinases; and (4) to determine the roles of the WD-repeat domain in MHCK A.