REGULATION OF REPRODUCTION

对建议的讨论再次强调了研究的优点 计划。然而，有人担心，拟议的工作可能没有 影响特别大。这个因素在一定程度上缓和了人们的热情。 描述(改编自申请人的摘要)：本申请 提出实验以检验类固醇激素合成是如何被控制的 在不同的类固醇生成组织中。为了做到这一点，调查人员 将重点研究调节大鼠P450c17中的一系列类固醇激素 细胞系。这些研究将使用体外转染法和凝胶移位法。 化验。在目标1中，研究人员将从 啮齿动物间质肿瘤细胞和肾上腺皮质细胞系与 大鼠P450c17基因中的调控元件。在具体目标2中，他们将 克隆这些因素。在具体目标3中，调查人员将探索 SF-1磷酸化在调节cAMP和/或基础P450c17基因中的作用 表情。

Discussion of the proposal re-emphasized the strong points of the research plan. However, there was some concern that the proposed work may not have a particularly high impact. This factor somewhat moderated enthusiasm DESCRIPTION (adapted from the applicant's abstract): This application proposes experiments to examine how steroid hormone synthesis is controlled in different steroidogenic tissues. To accomplish this, the investigators will focus on the regulation of rat P450c17 in a series of steroidogenic cell lines. These studies will use in vitro transfection and gel shift assays. In Aim 1, the investigators will identify nuclear proteins from rodent Leydig tumor cell and adrenocortical cell lines that interact with regulatory elements in the rat P450c17 gene. In Specific Aim 2, they will clone these factors. In Specific Aim 3, the investigators will explore the role of SF-1 phosphorylation in mediating cAMP and/or basal P450c17 gene expression.