BASEMENT MEMBRANE REGULATION OF SERTOLI CELLS

描述（改编自研究者摘要）：本申请 提出曲细精管的基膜成分 在支持细胞功能和形成一个决定性作用， 通过程序性细胞死亡的选择性过程， 审查 对于这些可能性，将采取三种办法。 具体目标1： 使用基底膜组分作为生精索的体外模型 将使用培养基质。 在具体目标2中，将重点研究 基底膜损伤后的信号转导机制 这些组分与支持细胞表面上的整合素结合。 一个主要 重点将是Ca 2+作为第二信使和原癌基因的作用 c-fos作为第三信使在睾丸支持细胞对高密度脂蛋白的反应中的作用 基底膜 在具体目标3中，基底膜作为 将研究支持细胞的存活因子，特别是 与Sertoli有关的曲细精管腔形成机制 细胞分化及其可能的程序性细胞死亡。

DESCRIPTION (Adapted from the Investigator's Abstract): This application proposes that basement membrane components of the seminiferous tubules play a determining role in Sertoli cell function and in the formation of a tubular lumen by a selective process of programmed cell death. To examine these possibilities, three approaches will be taken. In Specific Aim 1, an in vitro seminiferous cord model using basement membrane components as a culture substrate will be used. In Specific Aim 2, studies will be focused on the mechanism of signal transduction that occurs after basement membrane components bind to integrins on the surface of Sertoli cells. A major emphasis will be the role of Ca 2+ as a second messenger and protooncogene c-fos as a potential third messenger in Sertoli cell responses to the basement membrane. In Specific Aim 3, the role of the basement membrane as a survival factor for Sertoli cells will be studied, in particular the role and mechanism of seminiferous tubular lumen formation related to Sertoli cell differentiation and its possible programmed cell death.