WOUND HEALING MODEL USING ENDOTHELIAL CELL CULTURES

组织因子是由内皮细胞产生和释放的， 损伤，并且在凝血级联反应的启动中是重要的。 目前还不清楚与发热性疾病相关的体温升高， 可以影响组织因子的表达。 在这项研究中，我们检查了 人内皮静脉细胞表达组织因子 在热休克期间。 HUVEC暴露于44 ℃热休克0，1，2，4， 或8h。 组织因子和热休克蛋白72特异性mRNAs， 在加热1、2、4或8小时时使用核糖核酸酶保护试验检测 冲击. 热休克HUVEC表达升高水平的蛋白72特异性 mRNA在2小时达到峰值。 组织因子特异性mRNA 在未休克的HUVEC中响应于LPS而上调。 然而热 休克的HUVECs表达的TF mRNA水平降低 在热休克期间。 这些定性数据表明，转录 HUVECs中热休克蛋白和组织因子的调节不同 mRNA对热休克的反应。 组织因子表达调控 会受到热休克的影响 关键词：创伤愈合，内皮细胞，差异显示， 感染性心内膜炎

Tissue factor is produced and released by endothelial cells soon after injury and is important in the initiation of the coagulation cascade. It is unclear if elevated temperatures, associated with febrile illness, can affect tissue factor expression. In this study, we examined the expression of tissue factor by human endothelial vein cells (HUVECs) during heat shock. HUVECs were exposed to 44C heat shock for 0, 1, 2,4, or 8h. Tissue factor and heat shock protein 72-specific mRNAs were detected using ribonuclease protection assay at 1, 2,4, or 8h of heat shock. Heat shocked HUVECs express elevated levels protein 72 specific mRNA which peaked at 2 hours. Tissue factor specific mRNA was upregulated in unshocked HUVECs in response to LPS. However heat shocked HUVECs expressed reduced levels of TF mRNA in response to LPS during heat shock. These qualitative data suggest that transcriptional regulation in HUVECs differs for heat shock protein and tissue factor mRNA in response to heat shock. Regulated expression of tissue factor is affected by heat shock. Key words: wound healing, endothelial cell, differential display, infective endocarditis