GENETIC ANALYSIS OF NUCLEAR MIGRATION IN YEAST

描述：这项建议涉及Kip3，一种新的激动素，从 酵母数据库，在此之前已经涉及到核定位 后期。Kip3在星形小管上的定位已经被证明。 广泛的显微镜计划用来检验这一假说 用来正确定位纺锤体的星体小管。各种各样的 基因筛查计划是为了寻找可能起到偶联作用的蛋白质 Kip3连接到细胞皮质，从而使其能够正确定位细胞核 通过它对星体小管的作用。与小管的相互作用和与 相关蛋白质将通过生物化学进行探索。

DESCRIPTION: This proposal concerns Kip3, a new kinesin identified from the yeast database which has been implicated in nuclear orientation prior to anaphase. Localization of Kip3 to astral tubules has been demonstrated. Extensive microscopy is planned to test the hypothesis that it is these astral tubules that serve to correctly orient the spindle. A variety of genetic screens are planned to find the proteins that may function to couple Kip3 to the cell cortex and thereby allow it to correctly orient the nucleus through its action on astral tubules. The interaction with tubules and with associated proteins will be explored biochemically.