LIPID & PROTEIN TRAFFIC & DYNAMICS IN EPITHELIAL CELLS

上皮细胞，占体内所有细胞的50%[和 占所有人类癌症的90%]具有定义功能 创建和维护身体的生理隔间。 上皮细胞不仅在物理上分隔不同的环境； 它们监管它们之间的选择性交换。这就要求 细胞的相对表面在功能上被区分；因此， 上皮细胞在功能上被极化。这种极化是 通过协同编队和动态维护实现 相邻细胞之间的连接复合体和选择性或 膜蛋白和脂类的不同递送和保留 每个细胞的一极或另一极。本计划中的项目 向根尖和基底外侧选择性输送成分的研究 膜结构域。我们采取了不同的观点，不同的观点 技术被使用，但同时有一个共同的关注点 高尔基建筑群上的所有四个项目，考虑到它的横向 组织和异构性、流量、组成和完整性 并保持其空间方向。埃迪丁(项目1) 重点介绍高尔基体膜和血浆的侧向组织 膜蛋白，特别是GPI蛋白和脂类是必需的 这些分子的极化流量。哈伯德(项目3) 重点介绍极化的细胞内路径和机制 膜蛋白的传递和保留，重点是 内体/跨细胞系统。Machamer(项目4)专注于 高尔基复合体的组织和组成机制 这对于将脂类和蛋白质分选到 质膜，同时保持自身的完整性。施罗尔 (项目6)专注于维持细胞骨架的机械 上皮细胞的极化细胞内结构和在 小泡在细胞膜之间和细胞膜之间通过的路线 细胞质和表面。

Epithelial cells, which comprise 50% of all cells in the body [and account for 90% of all human cancers] have as a defining function the creation and maintenance of physiological compartments of the body. Epithelial cells not only physically separate different environments; they regulate selective exchange between them. This requires that the cells' opposing surfaces be functionally differentiated; hence, epithelial cells are functionally polarized. This polarization is accomplished through cooperative formation and dynamic maintenance of junctional complexes between adjacent cells and by the selective or differential delivery and retention of membrane proteins and lipids to one pole or the other each cell. The projects in this program study selective delivery of components to apical and basolateral membrane domains. A variety of perspectives are taken and different techniques are used, but at the same time there is a common focus in all four projects on the Golgi complex, considering its lateral organization and heterogeneity, traffic, composition, and integrity and maintenance of its spatial orientation. Edidin (Project 1) focuses on the lateral organization of Golgi membrane and plasma membrane proteins, particularly GPI-proteins, and lipids, required for the polarized traffic of these molecules. Hubbard (Project 3) focuses on intracellular routes and mechanisms for the polarized delivery and retention of membrane proteins, with emphasis on the endosomal/transcytotic system. Machamer (Project 4) focuses on the mechanisms of the organization and composition of the Golgi complex that important for sorting lipids and proteins destined for the plasma membrane, while maintaining its own integrity. Schroer (Project 6) focuses on the cytoskeletal machinery that maintains the polarized intracellular architecture of epithelial cells and on the routes traveled by vesicles between cytoplasmic membranes and between cytoplasm and surface.