HIGH PERFORMANCE QUADRUPOLE MS/MS SYSTEM

辛辛那提大学申请资金购买高性能三级四质谱计/质谱仪(MS/MS)系统，以升级其现有能力。该大学是美国历史最悠久的第十二所高等教育机构，也是俄亥俄州的两所综合性大学之一。凭借其在各级研究和教育方面的现代化方法，它提供了一批训练有素的科学家、医生和技术支持人员，目前在全国各地雇用。物理和生命科学学院处于许多科学和医学领域的前沿。然而，大学的MS设施没有跟上最先进的技术，这严重阻碍了这些研究人员的生产力。目前，该大学还没有类似于高性能三级MS/MS系统的仪器。在生物科学、化学、环境健康、分子遗传学、儿科学、药理学、药剂学和外科的研究和培训计划中，这种级别的MS分析是至关重要的。该仪器对于在NIH资助的项目中进行正在进行的实验以及在生物降解和生化毒理学、毒素暴露的生物转化和生物标记物、致病微生物的脂质生物化学和生理学以及蛋白质肽结构和化学等领域的未来研究至关重要。该仪器还将用于各学科的研究生和博士后培训。像辛辛那提大学这样规模和资金水平的第一研究大学，如果没有这种手段，就不可能在实现其长期教育和高级研究目标方面保持竞争力。所要求的仪器将提高灵敏度和探测水平，并降低噪音水平，这是该大学现有仪器无法达到的特点。该仪器将能够提供大生物分子的质谱学数据。电离样品引入方法将使热不稳定的大而完整的生物分子的分析成为可能。目前可用的仪器所需的大分子的水解和衍生化可以改变这些分子的结构，因此科学期刊越来越要求完整的分子分析。所要求的仪器可以检测由高效离子电离方法形成的正离子或负离子。这将提高开展研究的能力，例如识别标志性脂质的结构，并使随后能够灵敏地检测人体和室外环境中存在的病原体和毒素降解物。该仪器将能够分析多环芳烃-DNA加合物等络合物，并有助于阐明酶、泵、药物和受体的活性部位。这些数据可能导致新药及其衍生物的开发和环境污染物生物降解产物的识别。整个大学有几个正在进行的项目，只有通过更新的MS能力才有可能完成或推进；除了目前可用的磁性部门双聚焦气相色谱/质谱仪。

The University of Cincinnati requests funds to purchase a high- performance triple-stage quadruple mass spectrometer/mass spectrometer (MS/MS) system to upgrade its existing capabilities. The University is the twelfth oldest higher educational institutional in the country, and is one of two comprehensive universities in the State of Ohio. With its modern approach to research and education at all levels, it has provided a pool of well-trained scientists, doctors and technical support people now employe across the country. The faculty in the physical and life sciences are at the forefronts of many areas of science and medicine. However, the MS facilities at the University have not kept up with state of the art technology, which is severely hampering the productivity of these researchers. There is currently no instrumentation at the University equivalent to the high-performance triple-stage MS/MS system. This level of MS analysis is critically needed for research and training programs in biological sciences, chemistry, environmental health, molecular genetics, pediatrics, pharmacology, pharmacy, and surgery. The instrument is essential to perform ongoing experiments in NIH-funded projects as well as a future research in fields such as biodegradation and biochemical toxicology, biotransformation and biomarkers of toxin exposure, lipid biochemistry and physiology of pathogenic microbes, and protein peptide structure and chemistry. This instrument will also be used for graduate student and post-doctoral training in various disciplines. A Research I university of the size and funding level of the University of Cincinnati cannot remain competitive in attaining its long-term educational and advanced research goals without such instrumentation. The requested instrumentation will enhance sensitivity and detection levels, and reduce noise levels, features which current instruments at the University cannot attain. The instrument will be able to provide mass spectral data for large biomolecules. The ionization dn sample introduction methods will enable the analysis of heat-labile large and intact biomolecules. Hydrolysis and derivitization of large molecules required by currently available instrumentation can alter the structure of these molecules, hence scientific journals are increasingly demanding intact molecule analyses. The requested instrument can detect either positive or negative ions formed by highly efficient ion ionization methods. This will improve the ability to conduct studies such as the structural identification of signature lipids and enable subsequent sensitive detection of pathogens and toxin degraders present in humans and the outdoor environment. The instrument will be able to analyze complexes such as polycyclic aromatic hydrocarbon-DNA adducts, and aid in the elucidation of active sites of enzymes, pumps, drugs and receptors. These data may lead to the development of new drugs and their derivatives and the identification of biodegradation products of environmental pollutants. There are several ongoing projects across the University that are only possible to complete or advance with updated MS capabilities; beyond the magnetic sector double-focusing gas chromatography/mass spectrometers currently available.