TANDEM MASS SPECTROMETER FOR BIOMOLECULAR ANALYSIS

俄克拉荷马州大学的化学和生物化学系提出了一种具有大气压电离（API）源的串联质谱仪，该质谱仪能够进行电喷雾电离（ESI）和大气压化学电离（APCI）。该仪器将成为化学和生物化学部门已建立的分析服务中心的一部分。这种新仪器将支持俄克拉荷马州大学越来越多的生物无机、生物有机和生物化学研究小组的活动。该仪器的主要使用者将是本提案中包括的NIH支持的用户组，尽管其他用户可以在有时间的基础上提交样本进行分析。该质谱仪将满足各种项目的质量分析需求，包括研究生物胺神经递质的不寻常氧化代谢物在异常脑化学中的作用，鉴定细菌铁载体，确定含Ru或Os的金属卟啉的物理结合供体，确定蛋白质和RNA核苷酸相互作用的结合位点，以及确定酶的自杀失活的机理和化学计量。

The Department of Chemistry and Biochemistry at the University of Oklahoma proposes the acquisition of a tandem mass spectrometer with an atmospheric pressure ionization (API) source capable of both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). The instrument will become a part of the established Analytical Services Center in the Chemistry and Biochemistry Department. This new instrument will support the activities of a growing number of bioinorganic bioorganic and biochemical research groups at the University of Oklahoma. Primary use of this instrument will be by the NIH-supported user group included in this proposal, although other users may submit samples for analysis of a time-available basis. This mass spectrometer will fill the mass analysis needs for a variety of projects including investigations into the roles of unusual oxidative metabolites of biogenic amine neurotransmitters in aberrant brain chemistry, identification of bacterial siderophores, determination of physical binding donors to metalloporphyrins containing Ru or Os, determination of the binding site for interaction of protein and RNA nucleotide, and the determination of the mechanism and stoichiometry of suicide inactivation of enzymes.