FINNIGAN LCQTM BENCHTOP MS SYSTEM FOR HPLC

该提案要求支持购买LC/MS系统，以增强罗斯韦尔公园癌症研究所质谱设施的能力。所要求的仪器是Finnigan LCQ/TM离子阱台式质谱仪，配有电喷雾（ESI）和大气压化学电离（APCI）源。还寻求资金用于匹配的HPLC系统、具有UV/维斯检测器和膜脱气器的P4000-040四元梯度系统（来自Thermo Separation Products，NJ）和纳米电喷雾离子源（Protein Analysis Company，Odense，丹麦）。Finnigan LCQ/TM系统具有0-2000道尔顿质量范围分析仪和LC/MS/MS/n能力。该系统由一台装有Microsoft Windows NT操作系统的PC机控制。生物化学软件用于ESI操作的多电荷离子包被的分子量测定、蛋白水解酶的肽图谱以及肽序列预测和构象。Finnigan LCQ/TM LC/MS系统将为11名NIH资助的研究人员和2个NIH资助的研究小组提供急需的分析服务。它将用于通过蛋白质和肽的分子量、氨基酸序列、二硫键以及共价和其他修饰来表征蛋白质和肽。该系统还将用于研究寡核苷酸、寡糖、药物代谢物、核酸代谢物等。LCQ/TM系统将允许使用LC/MS进行定性和定量研究，该技术目前在RPCI不可用。通过MS/MS技术使用选择离子监测的LC/MS对于药物代谢物、氧化损伤的寡核苷酸、核酸代谢物等的在线定量将是重要的，在复杂的生物基质中含量很低。

This proposal requests support for the purchase of an LC/MS system to augment the capabilities of the Mass Spectrometry Facility at Roswell Park Cancer Institute. The instrument requested is a Finnigan LCQ/TM ion trap benchtop mass spectrometer with electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources. Funds are also sought for a matching HPLC system, P4000-040 quaternary gradient system with UV/VIS detector and membrane degasser (from Thermo Separation Products, NJ) and a nanoelectrospray ion source (Protein Analysis Company, Odense, Denmark). The Finnigan LCQ/TM system has a 0-2000 dalton mass range analyzer and LC/MS/MS/n capabilities. The system is controlled by a PC with Microsoft Windows NT operating system. The biochemistry software is used for molecular weight determination from multi-charged ion envelope operated by ESI, peptide mapping of proteolytic digests and peptide sequence prediction and conformation. The Finnigan LCQ/TM LC/MS system will provide much needed analytical services to eleven NIH-funded investigators and two NIH funded research groups. It will be used to characterize proteins and peptide by their molecular weight, amino acid sequence, disulfide linkages and covalent and other modifications. This system will also be used to study oligonucleotides, oligosaccharides, drug metabolites, nucleic acid metabolites, etc. The LCQ/TM system will permit qualitative and quantitative studies using LC/MS, a technique not currently available at RPCI. LC/MS using selected ion monitoring via MS/MS techniques will be important for on-line quantitation of drug metabolites, oxidatively damaged oligonucleotides, nucleic acid metabolites, etc., at very low levels in complex biological matrices.