MULTIUSER BIOLOGICAL TRANSMISSION ELECTRON MICROSCOPE

电子显微镜是一项非常重要的技术 分子和细胞生物学。我们对细胞的大部分了解 超微结构通过电子显微镜观察。最近， 有几项进步使电子显微镜的使用成为可能。 获得超越描述性信息的洞察力和 电子显微镜已成为研究分子的有力工具 机械装置。在很大程度上，这些发展反映了 遗传方法的高灵敏免疫细胞化学技术 扰乱单个蛋白质的功能，以及细胞自由的功能 使分析孤立的细胞器或 大分子复合体。此外，这方面的进步 低温电子显微镜正在缩小经典细胞之间的差距 生物学和结构生物学。 本申请建议购买新的传输电子产品 显微镜(飞利浦CM120 BioTwin)及配套设备数字化 成像。这个仪器将被安置在电子设备中。 耶鲁大学细胞成像中心的显微镜单元 医学。这是一个共享设施，由 细胞生物学系，但由学校资助。它是 主要由细胞生物学系的教职员工和 其他专注于分子和细胞研究的部门 生物学。该仪器将取代JEOL 100cX 最近退役 耶鲁大学的电子显微镜核心设备传统上是 在电子显微镜应用方面处于领先地位 生物和医学。虽然这种情况继续存在，但进展 现在正受到显微镜供应和过时的 设施中存在的仪器。已经有了重大的 电子显微镜设计和图像数字化的研究进展 该设施迫切需要一台最先进的显微镜。这个 耶鲁大学细胞生物学的生产力关键取决于 这类仪器的可用性。

Electron microscopy is a technique of fundamental importance in molecular and cell biology. Most of what we know about cell ultrastructure was learned from electron microscopy. Recently, several advancements have made possible he use of electron microscopy to obtain insight which go beyond descriptive information and the electron microscope has become a powerful tool to study molecular mechanisms. In large part, these developments reflect the advent of highly sensitive immunocythochemical techniques, of genetic methods to disrupt the function of individual proteins, and of cell free systems which make possible the analysis of isolated organelles or macromolecular complex. Furthermore, the advancements in cryoelectron microscopy are narrowing the gap between classical cell biology and structural biology. This application proposes the purchase of new transmission electron microscope (Philips CM120 BioTwin) and supporting equipment digital imaging. This instrumentation will be housed in the electron microscopy unit of the Center for Cell Imaging at the Yale School of Medicine. This is a shared facility, which is managed by the Department of Cell biology, but is subsidized by the School. It is used primarily by faculty from the Department of Cell Biology and other Departments whose research focuses on molecular and cell biology. The instrument will replace a Jeol 100cX which has been recently taken out of service The Yale Electron Microscopy Core Facility has traditionally been among the leaders in the application of electron microscopy to biology and medicine. While this continues to be the case, progress is now being hampered by microscope availability and by the outmoded instrumentation present in the facility. There have been major advances in electron microscope design and image digitalization and the facility is in urgent need of a state-of-the-art microscope. The productivity of Yale Cell Biology is crucially dependent on the availability of such an instrument.