IMAGE PLATE DETECTOR AND X RAY GENERATOR

明尼苏达大学结构生物学中心的蛋白质结晶学部门申请资金购买一台X射线发生器和一台图像板探测器。结构生物学中心为来自至少七个部门的两个校区的研究人员提供服务。四个系的五名教员使用这些资源和X射线结晶学作为他们进行结构：功能研究的主要工具。目前，联邦政府资助的15个研究项目直接依赖于X射线衍射设备。目前的仪器已有近十年的历史，必须在不久的将来更换。在一些研究中，现有设备没有提供项目所需的分辨率限制，超过一半的同时出现的反射没有被记录下来。1989年，明尼苏达大学利用明尼苏达医学基金会和大学运营基金的资金建立了蛋白质结晶学资源。没有使用NIH或其他联邦资金。在过去的十年里，来自明尼苏达大学的资金来源通过提供一套新的实验室和高性能的计算资源来解决特别困难的结晶学问题，从而使该中心的进一步发展成为可能。此外，目前结晶学工作人员使用的大多数图形工作站都是用大学的资金购买的。目前的资源在脂类：蛋白质相互作用、金属酶、铁储存蛋白、柠檬酸循环酶、微生物毒素和酶的代谢控制等领域取得了一些重要进展。目前，包括博士后研究员和研究生在内，大约有20名科学家使用X射线资源作为他们正在进行的研究的一部分。此外，一些合作项目将需要使用x射线衍射仪。

The Protein Crystallography Division of the Structural Biology Center at the University of Minnesota requests funds to purchase an x-ray generator and an image plate detector. The Structural Biology Center services investigators on two campuses from at least seven departments. Five faculty members in four departments use the resources and x-ray crystallography as their principal tool for structure:function studies. Currently 15 federally funded research programs are directly dependent on the x-ray diffraction equipment. The present instrumentation is nearly ten years old and must be replaced in the immediate future. In a number of studies, the existing equipment does not provide the resolution limits required by the project and more than half of the simultaneously appearing reflections are not recorded. The University of Minnesota established the Protein Crystallography Resource in 1989 with funds from the Minnesota Medical Foundation and from the University's operating funds. No NIH or other federal funds were used. During the last decade, monetary sources from the University of Minnesota have made possible the further development of the Center by providing a new suite of laboratories and high-powered computing resources for especially difficult crystallographic problems. In addition, most of the present graphics workstations used by the crystallographic staff have been purchased with University funds. The current resources have produced a number of important advances in areas such as lipid:protein interactions, metalloenzymes, iron storage proteins, citric acid cycle enzymes, microbial toxins and metabolic control of enzymes. At the present time and including post-doctoral fellows and graduate students, approximately twenty scientists use the x-ray resources as part of their ongoing research. In addition, a number of collaborative projects will need access to the x-ray diffraction instrumentation.