CORE--TISSUE CULTURE

SDRC组织培养核心实验室收到了大量的 在过去的四年里， 调查人员利用其服务。 实验室服务于 有效地促进获得、制备和培养 组织和细胞，这是至关重要的，但耗时， 昂贵的皮肤相关研究。 而且 实验室已成为讨论的焦点， SDRC调查人员，直接导致了几个新的合作 研究项目，解决皮肤生物学和疾病的 皮肤 目标是：1）协助应用现代技术 和方法，以皮肤相关的研究， 组织和细胞; 2）通过以下方式降低皮肤相关研究的成本： 组织/细胞获取的系统化和同步化， 培养过程; 3）确保组织和细胞的质量， 研究和研究人员的安全; 4）促进进一步的相互间- 部门讨论和协作，以皮肤为导向 5）将我们获得的知识应用于基础研究 以及人类皮肤病的临床问题。 实现 这些目标我们将继续：1）作为一个可靠的来源， 皮肤组织（人和小鼠皮肤库），2）纯化和培养 来自人类和小鼠皮肤的不同细胞群，包括 来自皮肤病患者的活检标本（细胞库），3） 以具有成本效益的方式提供广泛的单克隆 不同细胞类型的抗体和生长因子（抗体 和生长因子银行），4）购买组织培养试剂， （5）建立共享机制， 组织和细胞的质量，并）提供分离和 从皮肤培养细胞。 这些服务应该促进皮肤- 高质量的研究，减少时间， 开销. 正如这个实验室的广泛使用所证明的那样， 对服务的需求不断增长， 组织培养核心是必不可少的持续发展， SDRC。

The SDRC Tissue Culture Core Laboratory has received heavy and enthusiastic use during the past four years, with more than 40 investigators utilizing its services. The laboratory has served effectively in facilitating the acquisition, preparation, and culturing of tissue and cells, which are critical, yet time-consuming and expensive aspects of skin-related research. Moreover, the laboratory has served as a focal point for discussions among SDRC investigators, leading directly to several novel collaborative research projects that address skin biology and diseases of the skin. Objectives are to: 1) assist in applying modern technologies and methodologies to skin-related research by providing skin tissue and cells; 2) reduce the costs of skin-related research by systematizing and synchronizing tissue/cell procurement and culturing processes; 3) ensure the quality of tissues and cells being studied, and the safety of investigators; 4) promote further inter- departmental discussion and collaboration on skin-oriented research; and 5) apply the knowledge that we gain to fundamental and clinical questions about skin disease in humans. To achieve these goals we will continue to: 1) serve as a reliable source of skin tissues (Human and Mouse Skin Banks), 2) purify and culture different cell populations from human and mouse skin, including specimens biopsies from patients with skin disease (Cell Bank), 3) provide, in a cost-effective manner wide panels of monclonal antibodies and growth factors for different cell types (Antibody and Growth Factor Banks), 4) purchase tissue culture reagents in bulk quantities and to develop sharing mechanisms, 5) monitor the quality of tissues and cells, and ) provide training in isolating and culturing of cells from skin. These services should promote skin- oriented research of high quality and with reduced time and expense. As evidenced by the extensive use of this laboratory and the growing need for services, the continued support for the Tissue Culture Core is essential for the continued progress of the SDRC.