ASSEMBLY OF AN AMINOACYLATING RIBONUCLEOPROTEIN

能够进行RNA氨酰化的核糖核蛋白（RNP）催化剂将 集合。 该RNP将基于丙氨酰-tRNA的截短形式 合成氨酰-AMP但缺乏RNA识别的合成酶 域 因此，将采用体外选择方法来获得 合适的RNA适体，其将重新建立重组蛋白的能力。 催化剂结合RNA底物。氨酰化效率为 由此产生的RNP将被研究，RNA-蛋白质界面 将使用生物化学方法表征复合物中存在的 技术. 有人提出核糖核蛋白是常见的 催化剂在进化期间桥接RNA和蛋白质- 这是一个很大的问题，也是一个很大的问题。 由于它们在蛋白质中的重要作用， 合成. 因此，氨酰化RNP的组装将提供 合成酶可能进化前体的证据， 含有RNA成分。

A ribonucleoprotein (RNP) catalyst capable of RNA aminoacylation will be assembled. This RNP will be based on a truncated form of alanyl-tRNA synthetase which synthesizes aminoacyl-AMP, but lacks an RNA recognition domain. Hence, in vitro selection methods will be employed to obtain a suitable RNA aptamer that will re-establish the ability of the catalyst to bind an RNA substrate. The aminoacylation efficiency for the resultant RNP will be investigated and the RNA-protein interfaces present within the complex will be characterized using biochemical techniques. It has been proposed that ribonucleoproteins were common catalysts during the period of evolution bridging RNA and protein- dominated worlds, and it is likely that the synthetases appeared very early in this transition because of their important role in protein synthesis. Thus, the assembly of an aminoacylating RNP would provide evidence for possible evolutionary precursors to the synthetases that contained RNA components.