INDUCTION OF CERVICAL RIPENING W/ INTERLEUKIN 8 IN PREGNANT RHESUS MONKEYS

白介素8(IL-8)是一种诱导选择性 中性粒细胞的趋化和活化。黄体酮抑制和 抗孕激素通过绒毛和蜕膜刺激IL-8的产生。 宫颈成熟与炎症细胞的涌入有关 但IL-8在灵长类分娩中的作用仍有待于 被建立起来。为了研究IL-8在宫颈成熟中的作用， 我们将纯化的人成纤维细胞来源的IL-8经阴道注射 在3只怀孕的猴子中，从怀孕137-9天开始(足月=167 天)，剂量为150 ng/kg。宫颈采用改良的宫颈测定仪进行评估。 毕晓普=S得分。IL-8在车载(2.5%HPC，300 ？L·埃文斯蓝溶液)将1.0ml或3.0ml注射器插入 阴道穹隆。每隔一段时间进行羊膜穿刺术，以确定 羊水前列腺素(PG)和细胞因子的变化。在.之后 恢复期4-5天，第二次注射IL-8(300 ng/kg) 管理。结果分级剂量阴道给药效果观察 修正毕晓普的IL-8=S得分(均值？扫描电子显微镜)已在 下表。每三只动物中就有两只羊水含量增加 羊水中IL-8浓度无明显变化 PGE2或PGF2？一只动物在第二次注射IL-8后出生。 剂量0小时24小时48小时72小时150 ng/kg 0.6？0.3 5.6？0.3* 5.0？1.2*5.3？0.3*300 ng/kg 2.0？1.0 4.0？3.0 6.5？0.5* *与P&lt；0.05的时间0小时不同(t检验)。结论 纯化的人成纤维细胞来源的IL-8阴道内应用 诱导妊娠晚期恒河猴显著的宫颈成熟。 资助NIH HD06159，东丽工业公司出版物Haluska GJ， 火影忍者M，艾达·N，库克MJ，诺维·MJ。宫颈成熟的诱导作用 白介素8(IL-8)对怀孕恒河猴的影响。在社会保护协会 第45届妇科调查计划与摘要年会 (1998年3月11日至14日在佐治亚州亚特兰大举行)，第190A页(摘要#T615)。

Interleukin-8 (IL-8) is a cytokine that induces selective neutrophil chemotaxis and activation. Progesterone inhibits and antiprogestins stimulate production of IL-8 by chorion and decidua. Cervical ripening is associated with an influx of inflammatory cells into the cervix but the role of IL-8 in primate parturition remains to be established. To investigate the role of IL-8 in cervical ripening, we administered purified, human fibroblast-derived IL-8 intravaginally in 3 pregnant monkeys beginning at 137-9 days of gestation (term = 167 days) at a dose of 150 ng/kg. The cervix was evaluated by a modified Bishop=s Score. IL-8 was administered in vehicle (2.5% HPC with 300 ?l Evans Blue solution) by inserting a 1.0 or 3.0 ml syringe into the vaginal vault. Amniocentesis was performed at intervals to determine changes in amniotic fluid prostaglandins (PGs) and cytokines. After a recovery period of 4-5 days, a second dose of IL-8 (300 ng/kg) was administered. Res ults The effect of graded doses of intravaginal IL-8 on Modified Bishop=s Scores (mean ? SEM) is summarized in the table below. Two out of three animals showed an increase in amniotic fluid IL-8 concentrations but there were no changes in amniotic fluid PGE2 or PGF2?. One animal delivered after the second dose of IL-8. Dose 0 hours 24 hours 48 hours 72 hours 150 ng/kg 0.6 ? 0.3 5.6 ? 0.3* 5.0 ? 1.2* 5.3 ? 0.3* 300 ng/kg 2.0 ? 1.0 4.0 ? 3.0 6.5 ? 0.5* *different from time 0 hrs at P<0.05 (t-test). Conclusion Intravaginal application of purified human fibroblast-derived IL-8 induces significant cervical ripening in late pregnant rhesus monkeys. FUNDING NIH HD06159, Toray Industries Inc PUBLICATIONS Haluska GJ, Naruto M, Ida N, Cook MJ, Novy MJ. Induction of cervical ripening with interleukin-8 (IL-8) in pregnant rhesus monkeys. In Society for Gynecological Investigation Program and Abstracts 45th Annual Meeting (held in Atlanta, GA, March 11-14, 1998), p 190A (abstract #T615).