TITRATION OF UNCLONED SIVPBJ14 VIRUS STOCK

许多SIV分离株已被鉴定，SIVPBj14在体外和体内都得到了很好的表征和研究。这种特殊的病毒分离株通常被认为比其他毒株如SIVmne和SIVmac251更具致病性。由于其致病性质，SIVPBj14可能更适合于猕猴的抗逆转录病毒研究，因为药物治疗的效果可以从病毒学和发病机制两个方面进行监测。出于这些原因，我们生产了大量无细胞的未克隆SIVPBj14病毒库，用于在猕猴模型中进行接种和抗逆转录病毒研究。该病毒群在猕猴体内进行滴定，以确定100%的感染量。进一步的滴定将是不必要的，所有未来的抗逆转录病毒药物研究都将对猕猴使用相同的病毒接种。1997年，8只接种了SIVPBj14的猕猴在长期跟踪研究中保持健康。

Many isolates of SIV have been identified and SIVPBj14 has been well characterized and studied both in vitro and in vivo. This particular viral isolate is generally considered to be more pathogenic than other strains such as SIVmne and SIVmac251. Because of its pathogenic nature, SIVPBj14 may be better suited to antiretroviral studies in macaques since the effects of drug therapy can be monitored on both virology and pathogenesis. For these reasons, we produced a large quantity of a cell-free uncloned SIVPBj14 virus stock for inoculations and antiretroviral studies in the macaque model. This virus stock was titrated in macaques to determine the 100% infectious dose. Further titrations will be unnecessary, and all future antiretroviral drug studies will use the same viral inoculum for the macaques. In 1997 eight macaques inoculated with SIVPBj14 remained healthy in a long-term follow-up study.