PURIFICATION OF NOVEL FACTOR INVOLVED IN PRODUCTION OF APO B48

现已证实，增加血浆胆固醇 这种水平与心脏病风险的增加有关。这个 血液中胆固醇的主要载体是一种名为 载脂蛋白B由于载脂蛋白B具有如此重要的生理作用，我们的 实验室正在研究这种蛋白质是如何产生的。有两个 血浆中apo-B的形式有大的apo-B100和小的apo-B100， Apo-B48，大约是Apo-B100的一半大小。Apo-B48是 在膳食胆固醇的吸收和运输中起着重要作用。我们 发现有一种不寻常的机制导致了 产生这种短形式的载脂蛋白B。我们的研究已经 我一直在生物化学和分子水平上研究这种机制 水平。我们已经发现，参与这一途径的因素之一 在狒狒肾脏中高水平表达。因此，我们正在 从鸵鸟肾中提纯这一因子以进行鉴定 并了解其在载脂蛋白B4 8合成中的作用。 我们的研究将提供对身体如何处理 饮食中的胆固醇。我们的长期目标是利用这些信息 设计可能有助于降低血液胆固醇水平和 预防未来动脉粥样硬化的发展。资助美国国立卫生研究院 授予HL45478和RR00166。Mehta，A.和D.M.Driscoll。一个 序列特异性RNA结合蛋白补充APOBEC-1编辑 载脂蛋白-B基因的表达。摩尔。牢房。生物，18 4426-4432,1998

It is now well established that increased plasma cholesterol levels are associated with an increased risk in heart disease. The main carrier of cholesterol in the bloodstream is a protein called apo-B. Because apo-B has such an important physiological role, our laboratory is studying how this protein is produced. There are two forms of apo-B in the plasma a large form, apo-B100, and a small form, apo-B48, which is about half the size of apo-B100. Apo-B48 is important in the absorption and transport of dietary cholesterol. We have found that there is an unusual mechanism responsible for the production of this short form of the apo-B protein. Our research has been investigating this mechanism at the biochemical and molecular level. We have found that one of the factors involved in this pathway is expressed at high levels in baboon kidney. Therefore, we are trying to purify this factor from baboon kidney in order to identify it and understand its role in the synthesis of apo-B4 8. The results from our research will provide insight into how the body handles dietary cholesterol. Our long-term goal is to use this information to design therapies that may help lower blood cholesterol levels and prevent the development of atherosclerosis in the future. FUNDING NIH grants HL45478 and RR00166. Mehta, A. and D.M. Driscoll. A sequence-specific RNA-binding protein complements apobec-1 to edit apolipoprotein-B mRNA. Mol. Cell. Biol., 18 4426-4432, 1998