Proteins in motion - new tools for biotechnology

运动中的蛋白质——生物技术的新工具

• 批准号: FL170100019
• 负责人: Prof Gottfried Otting
• 金额: $ 183.67万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Australian Laureate Fellowships
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-09-21 至 2022-09-20
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FL170100019
• 关键词: Proteins; motion; new; tools; biotechnology

This project aims to assess the function of proteins by monitoring their motions using new nuclear magnetic resonance (NMR) spectroscopy techniques. As snapshots of 3D protein structures have been determined by crystallography, the new tools are designed to analyse functionally important motions in solution. A facility for ultrafast (> 100 kHz) magic angle spinning NMR spectroscopy of proteins in the semi-solid state will bring cutting-edge know-how to Australia and allow the interrogation of 3D structure and dynamics in selected protein regions. The expected outcomes of the project will have immediate benefits for the rational engineering of biocatalysts and in the design of lead compounds in drug development.

该项目旨在通过使用新的核磁共振波谱技术监测蛋白质的运动来评估蛋白质的功能。由于3D蛋白质结构的快照已由结晶学确定，因此新工具旨在分析溶液中功能上的重要运动。用于半固态蛋白质的超快(&gt；100 kHz)魔角旋转核磁共振波谱的设备将为澳大利亚带来尖端技术，并允许询问选定蛋白质区域的3D结构和动力学。该项目的预期结果将对生物催化剂的合理设计和药物开发中的先导化合物设计产生立竿见影的效果。