REPRODUCTIVE PARAMETERS OF CHIMPANZEE MODEL

最近的数据表明，EP 1是一种胆固醇交换蛋白， 并且它由所有上皮细胞合成和分泌。 因此，我们认为， 我们集中精力确定EP 2在 受精过程 我们已经证明EP 2是一个 雄激素依赖性附睾特异性分泌蛋白， 合成和分泌，特别是在头区的 黑猩猩的附睾 我们已经确定了其他几种替代方案， EP 2消息，我们还没有特征。 此夕h 黑猩猩的选择性剪接模式不同于 恒河猴 恒河猴使用几个额外的外显子， 转录三个开放的阅读框架。 更好地了解 剪接变体和它们的两个启动子，我们开始研究一个基因组， EP 2克隆（PAK）。 PAK（P1人工染色体），具有插入大小 超过100 kb，似乎包含整个EP 2基因。 用PCR桥接内含子的初步分析表明， EP 2基因包含至少10个外显子，其中5个用于 在EP 2A变体中，最后3个用于EP 2B 变量。 我们已经证明，EP 1不结合精子表面。 从黑猩猩射出的精子中提取的蛋白质的蛋白质印迹显示， 仅在低盐提取物中强烈染色的组分。 使用 免疫组织化学染色，我们发现EP 1似乎是 合成和分泌主要由上皮VI-VIII的 中头通过远侧头近侧体的上皮IX 黑猩猩附睾管的过渡。 资助NIH /RR 0599407 $165，627 4/15/97 - 8/14/01出版物 *Smithwick，E.G.， Gould，K.G.和Young，L.G.的免疫组织化学定位 成年雄性黑猩猩附睾分泌蛋白EP 1（Pan troglodytes）。 组织和细胞（出版中）。 * 杨，L.G.，弗罗利希岛 和Gould，K.G. HE 2/EP 2，一种雄激素依赖性蛋白， 黑猩猩（Pan troglodytes）附睾。 J. Reprod. 费特 （在 Press）。 P51 RR 00165 -38 1998年1月1日-1998年12月31日Yerkes区域灵长类动物 研究中心

Recent data indicates that EP1 is a cholesterol exchange protein and that it is synthesized and secreted by all epithelia. Therefore, we have concentrated our efforts on determining the role of EP2 in the fertilization process. We have shown that EP2 is an androgen-dependent, epididymis-specific secretory protein that is synthesized and secreted specifically in the caput region of the chimpanzee epididymis. We have identified several other alternative EP2 messages, which we have not yet characterized. Further, the alternative splicing pattern for chimpanzee is different from that of the rhesus monkey. The rhesus monkey uses several additional exons to transcribe the three open reading frames. To better understand the splicing variants and their two promoters, we began work on a genomic EP2 clone (PAK). PAK (P1 artificial chromosome), has an insert size of over 100 kb that appears to contain the entire EP2 gene. Preliminary analyses, using PCR to bridge the intr ons, indi cates that the EP2 gene contains at least 10 exons, five of which are used in the EP2A variant and the last 3 of which are used in the EP2B variant. We have shown that EP1 does not bind to the sperm surface. Western blots of proteins from ejaculated chimpanzee sperm showed intensely stained components only in low salt extract. Using immunohistochemical staining, we showed that EP1 appears to be synthesized and secreted primarily by epithelia VI-VIII of the mid-caput through epithelium IX of the distal caput-proximal corpus transition of the ductus epididymides of the chimpanzee. FUNDING NIH / RR0599407 $165,627 4/15/97 - 8/14/01 PUBLICATIONS *Smithwick, E.G., Gould, K.G. and Young, L.G. Immunohistochemical localization of epididymal secretory protein EP1 in the adult male chimpanzee (Pan troglodytes). Tissue and Cell (In press). *Young, L.G., Frolich, O. and Gould, K.G. HE2/EP2, an androgen dependent protein of the chimpanzee (Pan troglodytes) epididymis. J. Reprod. Fert. (In press). P51RR00165-38 1/1/98 - 12/31/98 Yerkes Regional Primate Research Center