CORE--TRANSGENIC ANIMAL FACILITY

转基因动物设施为华盛顿大学癌症中心的工作人员提供 用于癌症的实验动物的基因组操纵能力 研究。高效的一代基因敲除或转基因动物 需要畜牧业领域的专门技能和 外科、胚胎学、胚胎干细胞培养和基因打靶 矢量学、胚胎显微操作和冷冻保存。此外, 这一过程需要敏感而昂贵的显微镜设备，因为 以及SPF-动物设施。因为这些要求不能通过 大多数个体研究人员在他们自己的实验室里，基因 靶向和转基因动物技术必须由共同 资源设施。在威斯康星大学，这一资源是 转基因动物设施(TAF)。在UWCCC进行的癌症研究 严重依赖于这一资源。为此，请拨出资金，以便 支持这一关键活动。

The Transgenic Animal Facility provides UW Cancer Center staff with the capacity to manipulate the genome of experimental animals used in cancer research. The efficient generation of knockout or transgenic animals requires specialized technical skills in the areas of animal husbandry and surgery, embryology, embryonic stem culture and gene targeting vectorology, embryo micro-manipulation, and cryopreservation. In addition, the procedures require sensitive and expensive microscopic equipment as well as SPF-animal facilities. Because these requirement cannot be met by most individual investigators in their own laboratories, the gene targeting and transgenic animal technologies must be provided by common resource facilities. At the University of Wisconsin, that resource is the Transgenic Animal Facility (TAF). The cancer research performed at UWCCC relies heavily upon this resource. For that reason, funds are requested to support this critical activity.