SALIVARY PROTEINS AND ORAL BACTERIAL ADHESION

我们过去在体外对模型系统的研究表明，特定的 唾液蛋白，吸附在合成羟基磷灰石(HA)上， 选择性促进几种主要口腔细菌的黏附 致医管局。这些发现强烈表明，特定的唾液蛋白， 吸附在牙齿上，可能在高选择性中起到重要作用 口腔细菌在牙齿上自然产生的初始粘连 表面。现在重要的是在活体内检验这一假设。使 为了提高这方面的知识，这项测试的目标如下 建议。目的1检验特定唾液的中心假说 吸附在牙釉质表面的蛋白质促进选择性 在体内，细菌附着在这些表面上。精选的纯样品 唾液蛋白，已知可促进口腔主要细菌的黏附 去透明质酸体外，将用于治疗釉质阻塞。这些区块将是 安装在假肢装置上，并将该装置放置在口腔中。这个 选定的细菌对蛋白质处理的牙釉质的初始黏附 在八个受试者的几个小时内通过组合的文化 以及使用16S rRNA探针进行DNA棋盘分析。微生物 粘附力数据将进行统计分析，以确定与 蛋白质前处理。目标2j检验一个受试者的 唾液蛋白质谱决定了膜的组成，而这 在体内影响细菌对牙釉质的选择性黏附。 唾液成分定义不清。因此，对水的吸附 特定的细菌对HA的黏附促进蛋白将是 在体外对目标1中使用的八个受试者进行量化，使用 为此目的开发的技术。因此， 不同的黏附促进蛋白将吸附在矿物上 下定决心。在目标1中获得的每个受试者的微生物黏附数据， 将被统计地分析与同一主题的关系 唾液蛋白质图谱和蛋白质吸附数据。目标3测试 假设一些唾液和血清蛋白质和细菌 葡聚糖和糖基转移酶等大分子起着 口腔细菌与透明质酸吸附唾液黏附的抑制剂 蛋白质。唾液和血清中的某些蛋白质和 选择细菌产品作为初始微生物黏附的抑制剂 是另一项必须系统量化的重要活动 了解在活体内选择性微生物对牙齿的黏附。因此， 唾液和血清的层析部分的影响，以及 特定细菌上的著名细菌大分子-唾液蛋白 黏附反应将在体外进行适当的测定，这些 数据将纳入对目标1结果的解释。

Our past studies in model systems, in vitro, have shown that specific salivary proteins, adsorbed onto synthetic hydroxyapatite (HA), selectively promote adhesion of several prominent oral bacterial species to HA. These findings strongly suggest that specific salivary proteins, adsorbed onto teeth, may play an important role in the highly selective initial adhesion of oral bacteria that occurs naturally on tooth surfaces. It is now important to test this hypothesis, in vivo. To make this test, and to advance knowledge in this area, the following aims are proposed. AIM 1 To test the central hypothesis that specific salivary proteins, adsorbed onto dental enamel surfaces, promote selective bacterial adhesion to those surfaces, in vivo. Pure samples of selected salivary proteins, known to promote adhesion of prominent oral bacteria to HA in vitro, will be used to treat enamel blocks. The blocks will be mounted on a prosthetic device, and the device placed in the mouth. The initial adhesion of selected bacteria to the protein-treated enamel will be determined over several hours in eight subjects by combined cultural and DNA checkerboard analyses using 16S rRNA probes. The microbial adhesion data will be analyzed statistically for relationships with the protein pretreatments. AIM 2 j To test the hypothesis that a subject's salivary protein profile determines pellicle composition, and that this influences selective bacterial adhesion to dental enamel in vivo. Salivary composition is poorly defined. Therefore, adsorption of specific bacterial adhesion-promoting proteins onto HA will be quantified, in vitro, for the eight subjects used in Aim 1, using techniques developed for that purpose. Thus, the amounts of the different adhesion-promoting proteins adsorbing onto the mineral will be determined. Each subject's microbial adhesion data, obtained in Aim 1, will be analyzed statistically for relationships with the same subject's salivary protein profile and protein adsorption data. AIM 3 To test the hypothesis that some salivary and serum proteins and bacterial macromolecules such as glucans and glycosyl-transferases act as inhibitors of adhesion of specific oral bacteria to HA-adsorbed salivary proteins. The possible role of some salivary and serum proteins and selected bacterial products as inhibitors of initial microbial adhesion is a further important activity that must be systematically quantified to understand selective microbial adhesion to teeth, in vivo. Therefore, the effects of chromatographic fractions from saliva and serum, and the noted bacterial macromolecules, on specific bacteria-salivary protein adhesion reactions will be determined, in vitro, As appropriate, these data will be incorporated into the interpretation of results of Aim 1.