REGULATION OF SODIUM, POTASSIUM, CHLORINE AND HYDROGEN TRANSPORT

我们的研究项目侧重于研究未解决的问题， 跨顶膜和基底膜的离子转运机制 有明确的小管节段。 我们将应用小管灌注和修补- 评价细胞和分子转运机制的钳夹技术 Na、K、C1、H等元素及其生理调节。 首先，我们提出探讨近端小管氯化物的机制 以顶端甲酸盐-氯化物和碳酸盐-氯化物为重点的运输 交易所 这些研究将针对回收的性质 甲酸盐和草酸盐的作用机理及阴离子的参与 近端小管NaCl生理调节中的交换体 运输 我们还将研究氯离子通道在 基底外侧膜介导净氯重吸收。 其次，我们建议研究Na，K，Ca和H转运的相互作用 在主小管细胞中。 我们将分析它们的酸碱转运 机制，并探讨其调节和与净钠的相互作用 钾的运输。 我们的研究将提供深入了解 “膜串扰”和影响Na、K、pH和Na+的信号传导途径 钙稳态。 第三，我们建议探索心尖低电导钾通道， 主小管细胞 我们将处理尚未解决的问题， 传导机制和生理调节。 我们还计划 探索最近克隆的ATP敏感通道并比较其性质 与主小管细胞中的天然K通道结合。

Our research project focuses on the study of unresolved problems of the mechanisms of ion transport across the apical and basolateral membranes of defined tubule segments. We shall apply tubule perfusion and patch- clamp techniques to evaluate cellular and molecular transport mechanisms of Na,K,C1 and H and their physiological regulation. First, we propose to explore the mechanism of proximal tubule chloride transport with emphasis o apical formate-chloride and oxalate-chloride exchange. These studies will be directed at the nature of the recycling mechanisms of formate and oxalate and the participation of the anion exchangers in the physiological regulation of proximal tubule NaCI transport. We shall also investigate the role of chloride channels in the basolateral membrane in mediating net chloride reabsorption. Second, we propose to study the interaction of Na,K,Ca and H transport in principal tubule cells. We shall analyse their acid-base transport mechanisms, and explore their regulation and interaction with net sodium and potassium transport. Our studies will provide insights into "membrane cross-talk" and the signaling pathways affecting Na,K,pH and Ca homeostasis. Third, we propose to explore the apical low-conductance K channel in principal tubule cells. We shall address unresolved problems of conduction mechanism and physiological regulation. We also plan to explore a recently clone ATP-sensitive channel and compare its properties with native K channels in principal tubule cells.