CANDIDA VIRULENCE AND HUMAN AGING

白色念珠菌是一种致病酵母菌，它作为一种共生菌存在于 大多数健康人的口腔。然而，在一个 易患条件数，白色念珠菌侵入组织引起 感染。人们一直认为，共生菌株是 随后的感染，并且几乎没有菌株专门化。 然而，这些假设最近受到了以下结果的质疑 证明不同的菌株在不同的解剖结构中携带 相同的健康女性的位置，该菌株的替换发生在 艾滋病患者的口腔和阴道炎患者的男性伴侣， 每个菌株都有一些通用的表型，受一个 可逆的高频开关机构，可以利用 在不同的环境条件下，这种污渍可以迅速 在基因水平上进化。因此，我们将测试基因 口腔共生菌与感染性菌株的关系 同一个人在从健康状态到假牙的转变中 口腔炎。在这项研究中，我们开发了一种计算机辅助DNA 使用适度重复的探针CA3的指纹系统，以及 分析表型转换的条件。此外，我们还将 比较来自相同个体的共生菌株和感染菌株的 推定的致病性状的数量。这项研究将在 一般级别1)是否涉及表型转换 从共生到病机的传递，以及4)是否共生 感染株的表型也不同。

Candida albicans is a pathogenic yeast which resides as a commensal in the oral cavity of a majority of healthy individuals. However, under a number of predisposing conditions, C. albicans invades tissue causing infection. It has been assumed that commensal strains are the source of subsequent infection and that there is little strain specialization. However, these assumptions have recently been questioned by results which demonstrate that different strains are carried in different anatomical locations of the same healthy women, that strain replacement occurs in the oral cavity of AIDS patients and male partners of vaginitis patients, that each strain has a number of general phenotypes, regulated by a reversible, high frequency switching mechanism, which may be utilized under different environmental conditions, and that stains can rapidly evolve at the genetic level. We, therefore, will test the genetic relationship between commensal and infecting strains of the oral cavity of the same individual in the transition from a healthy state to denture stomatitis. For this study, we have developed a computer-assisted DNA fingerprinting system employing the moderately repetitive probe Ca3, and conditions for analyzing phenotype switching. In addition, we will compare commensal and infecting strains from the same individuals for a number of putative virulence traits. This study will answer at the generic level 1) whether phenotypic switching is involved in the transmission from commensalism to pathogenesis, and 4) whether commensal and infecting strains differ in phenotype.