RNA STUDIES AND THE CREUTZFELDT-JACOB DISEASE AGENT

这个项目的最终目标是定义CJD代理特定的 (非宿主)分子，即蛋白质和核酸。它是 显然，对这些元素的定义将对 对这些代理人和他们的痴呆症的最终理解 归纳。对真正的药剂特定分子的鉴定将 还允许对人类痴呆症进行可靠的诊断测试。 该项目被分成两部分。第一个处理的是 对感染性疾病的大小和密度进行最终评估 为进一步的系统提纯奠定了基础 试剂，以及试剂蛋白的检测(S)。第二部分 专注于特定基因的检测、扩增和测序 可能属于感染性病原体的RNA(cdna克隆)。

The ultimate aim of this project is to define CJD agent specific (non-host) molecules, i.e. proteins and nucleic acids. It is obvious that definition of these elements will be invaluable for the ultimate understanding of these agents and the dementias they induce. The identification of truly agent specific molecules will also permit reliable diagnostic tests for human dementias. The project is split into two parts. The first deals with a definitive evaluation of the size and density of the infectious agent and will be useful for further systematic purification of agent, and detection of agent protein(s). The second part concentrates on detection, amplification and sequencing of specific RNAs (cDNA clones) that may belong to the infectious agent.