DIETARY PATTERNS ON SERUM OXYGEN RADICAL ABSORBING CAPACITY/LIPID PEROXIDATION

我们研究的总体目的是确定 饮食模式影响抗氧化能力和自由基介导的 细胞损伤 在一项临床试验中， 三种饮食中的一种， 和蔬菜. 自由基介导的损伤通过测量 的脂质过氧化作用，并与抗氧化能力的 血 我们的研究是作为一项辅助研究， 一项由NHLBI赞助的临床试验， 高血压 这项研究的设计是随机的，安慰剂- 对照临床试验。 志愿者参与者与边缘， 轻度高血压，无其他伴随疾病，有资格 本研究 该试验旨在观察饮食的影响， 不同的脂肪含量和数量的水果和蔬菜对血液 压力 有兴趣且符合条件的患者均被安置三周 饮食中脂肪含量为37%，水果和蔬菜含量较低。 后 三周后，患者被随机分为三种饮食， 基线饮食，低脂肪（25%）和高水果的饮食， 蔬菜，或饮食中有37%的脂肪，但水果含量高， 蔬菜. 试验的主要结果变量是血压 测量. 我们辅助研究的目的是确定饮食的影响 对 动脉粥样硬化前体的测量，包括 收集和 分析呼气乙烷和血清，以确定 脂质含量 过氧化作用 我们的假设是饮食 富含水果和蔬菜， 抗氧化剂，是保护免受自由基介导的损害， 通过脂质过氧化反应反映出来。结果变量也将是 与基线动脉粥样硬化性心血管疾病风险相关 因素 1996年6月，我们完成了五个队列中的最后一个。 有 在招募方面付出了巨大的努力，这是非常成功的。 我们 共招募并抽样了123名参与者，最后一个队列中有35名 在最近的财政年度。这些数字超过了我们的先验 期望，我们基于此计算功率。 所以我们有 完成了数据收集，我们目前正在进行数据分析 迄今为止，已提交了一份出版物， 准备.

The overall purpose of our research is to establish how different dietary patterns affect antioxidant capacity and free-radical mediated cellular damage. In a clinical trial, human volunteers are randomized to one of three diets that vary in fat content and amounts of fruits and vegetables. Free-radical mediated damage is assessed with measures of lipid peroxidation and is related to the antioxidant capacity of the blood. Our research has been conducted as an ancillary study within the context of an NHLBI-sponsored clinical trial entitled Dietary Approach to Stop Hypertension. The design of the study is a randomized, placebo- controlled clinical trial. Volunteer participants with borderline to mild hypertension, and no other concomitant disease, are eligible for this study. The trial was designed to look at the effects of diets that vary in fat content and the amounts of fruits and vegetables on blood pressure. Patients who are interested and eligible are all placed for three weeks on a diet that is 37% fat and is low in fruits and vegetables. After three weeks, patients were randomized to one of three diets, either the baseline diet, a diet that is low in fat (25%) and high in fruits and vegetables, or on a diet that has 37% fat but is high in fruits and vegetables. The main outcome variable for the trial is blood pressure measurements. The objective of our ancillary study is to determine the effects of diet on measures of the precursors of atherosclerosis, including the collection and analysis of breath ethane and serum to determine the extent of lipid peroxidation. Our hypothesis is that diets high in fruits and vegetables which have an abundance of exogenous antioxidants, are protective against free-radical mediated damage as reflected by peroxidation of lipids.. Outcome variables will also be correlated with baseline atherosclerotic cardiovascular disease risk factors. In June of 1996, we completed the last of our five cohorts. There was a tremendous effort in recruitment, which was highly successful. We enrolled and sampled 123 participants overall, 35 in the last cohort during the most recent fiscal year. These numbers exceed our a priori expectations, on which we based our power calculations. Hence we have completed data collection, and we are currently performing data analysis that to date has resulted in one submitted publication and one in preparation.