CORE--FLOW CYTOMETRY

该提案要求继续为流动方案提供部分资金 UAB艾滋病中心内的细胞计数核心设施（FACS核心）。的 FACS核心的主要目的是使艾滋病中心 调查人员的设备，训练有素的人员，和专业知识，以执行 使用最先进的设备进行细胞分析和纯化， 以合理的服务成本为基础的技术，并提供这些 能够处理潜在传染性的设施中的服务 材料外地资产管制系统核心每年为30多名调查员提供服务， 超过85%的实际预约时间被艾滋病中心成员使用。在 目前，核心包含一个FACStar单激光器四 参数，双色流式细胞仪/分选仪。在不久的将来，利用资金 从UAB卫生服务基金会获得，该仪器将 更换为FACStar/plus六参数双激光细胞计数器/分选仪。 多功能流式细胞仪的流式细胞仪和流式细胞仪 关节炎和肌肉骨骼疾病中心是联合经营的， 通过以下方式改进对调查员的服务 提高设备和人员的灵活性。两大核心设施 联合聘用两名有细胞计数经验人员 运营商要求提供资金，以确保稳定支持50%的 经营者的工资，经营者的继续教育， 细胞仪维护合同。所要求的支助水平将是 足以支付略高于50%的实际运营成本 FACS核心这将使目前艾滋病每小时50美元的费率 中心调查人员将在可预见的未来持续工作。

This proposal is a request for continued partial funding of the Flow Cytometry Core Facility (FACS Core) within the UAB AIDS Center. The primary purposes of the FACS Core are to make accessible to AIDS Center investigators the equipment, trained personnel, and expertise to perform cell analysis and purification with state-of-the-art equipment and techniques on a reasonable cost for services basis, and to offer these services in a facility capable of handling potentially infectious material. The FACS Core serves over 30 individual investigators per year, with over 85% of actual booked time being used by AIDS Center members. At the present time, the core contains a FACStar single laster four parameter, two color cytometer/sorter. In the near future, using funds obtained from the UAB Health Services Foundation, this instrument will be replaced with a FACStar/plus six parameter, dual laser cytometer/sorter. The FACS Core and the Flow Cytometry Core Facility of the Multipurpose Arthritis & Musculoskeletal Diseases Center are operated jointly, an arrangement which results in improved service to investigators through increased flexibility in equipment and personnel. The two core facilities jointly employ two individuals who are both experienced cytometer operators. Funds are requested to ensure stable support for 50% of the operators' salaries, continuing education for the operators, and the cytometer maintenance contract. The requested level of support will be sufficient to cover slightly more than 50% of the actual cost of operating the FACS Core. This will allow the present rate of $50/hour for AIDS Center investigators to be sustained into the foreseeable future.