IMMUNOLOGICAL, BIOCHEMICAL, AND MOLECULAR STUDIES ON EPITHELIAL OVARIAN CANCER

这个项目的主要目标是生成与生物学有关的数据 上皮性卵巢癌(EOC)，特别强调 沙门氏菌特异性抗原的鉴定与鉴定 肿瘤类型。正在进行的研究已经确定了四个抗原系统 与EOC特别相关：1)MX35抗原；2)CA-125抗原；3) 粘蛋白抗原，例如MUC-1；以及4)血型相关的特异性， 比如刘易斯。这个项目将强调详细的生化， 免疫化学，以及这些抗原的分子分析。MX35抗原有 被鉴定为95,000道尔顿的糖蛋白，CA-125为粘蛋白- 类型分子。其中一个目标是克隆编码这种蛋白质的基因 这些分子的一部分，目的是了解它们的分子 化妆，也许还有功能。对于粘蛋白抗原，我们计划在 详述MUC-1粘蛋白和CA-125的糖基化特性 抗原。我们的目标是确定糖基化如何对 针对这些抗原的肿瘤特异性抗体以及是否 粘蛋白糖基化是一种组织特异性特征。这些实验 也可以为EOC的免疫治疗指明新的靶点。决赛 目标将是确定多肽是否模拟碳水化合物表位 EoC的特性，例如Le/y，可以作为疫苗 抗肿瘤反应的发展。总体而言，该项目旨在 了解卵巢癌的免疫原性并利用它 开发新的治疗方法的信息。

The main goals of this project are to generate data concerning the biology of epithelial ovarian cancer (EOC) with a special emphasis on the identification and characterization of antigens characteristic of this tumor type. On-going studies have identified four antigenic systems particularly pertinent to EOC: 1) MX35 antigen; 2) CA-125 antigen; 3) mucin antigens, e.g., MUC-1; and 4) blood group-related specificities, such as Lewis. This project will emphasize the detailed biochemical , immunochemical, and molecular analysis of these antigens. MX35 antigen has been identified as a glycoprotein of 95,000 dalton and CA-125 as a mucin- type molecule. One goal will be to clone the genes coding for the protein portion of these molecules, with the aim of understanding their molecular makeup and perhaps function. For mucin antigens, we plan to analyze in detail the glycosylation characteristics of MUC-1 mucin and CA-125 antigen. The goal is to determine how glycosylation contributes to the tumor-specific of antibodies directed towards these antigens and whether mucin glycosylation is a tissue-specific characteristic. The experiments could also point towards new targets for the immunotherapy of EOC. A final goal will be to determine whether peptide mimics of carbohydrate epitopes characteristic of EOC, e.g., Le/y, can serve as vaccines for the development of anti-tumor responses. Overall, the project aims at understanding the immunogenicity of ovarian cancer and using this information to develop new therapeutic approaches.