IMMUNOLOGICAL, BIOCHEMICAL, AND MOLECULAR STUDIES ON EPITHELIAL OVARIAN CANCER

该项目的主要目标是鉴定抗原特性 上皮性卵巢癌（EOC）使用小鼠单克隆抗体 （mAb）并研究卵巢上皮生物学的某些方面， 它的恶性转化。 通过mAb检测的抗原将是 使用生物化学和分子方法表征。 一个重要 目的是确定适用于临床试验的mAb 涉及平等机会委员会。 待研究的mAb包括mAb MX35（检测95，000个 糖蛋白），mAb VK-5、6和7（检测MUC-1粘蛋白表位）， mAb MW 207（检测叶酸结合蛋白）和mAb VK-8（检测叶酸结合蛋白） 新EOC抗原）。 该项目还将测试嵌合和人源化 抗血型Le抗体的特异性， 其他参数。 另一个目标将是使用各种mAb来检测 与正常卵巢上皮进展为EOC相关的标志物 和适于卵巢肿瘤分类的标记物。 三 将检查抗原类型：1）血型抗原，2） 外肽酶和3）粘蛋白。 粘蛋白将被更详细地研究 利用生物化学和分子技术， 粘蛋白在EOC中发挥的作用。 此外，新的分析方法， 糖蛋白和粘蛋白的碳水化合物含量将被开发。 这些目标的实现，将为生物医学的发展提供新的试剂。 EOC的诊断和治疗，并将导致了解 与正常组织恶性转化相关的抗原变化 卵巢上皮，肿瘤进展，和生物学行为， 卵巢肿瘤

The major goal of this Project are to identify antigens characteristics of epithelial ovarian cancer (EOC) using mouse monoclonal antibodies (mAbs) and to study some aspects of the biology of ovarian epithelium and its malignant transformation. The antigens detected by the mAbs will be characterized using biochemical and molecular approaches. An important aim will be to identify mAbs that are suitable for use in clinical trials involving EOC. Among mAbs to be studied are mAb MX35 (detecting a 95,000 glycoprotein), mAbs VK-5, 6, and 7 (detecting the MUC-1 mucin epitope), mAb MW 207 (detecting folate-binding protein) and mAb VK-8 (detecting a novel EOC antigen). This Project will also test chimeric and humanized versions of an anti-blood group Le antibody for their specificity and other parameters. Another goal will be to use various mAbs to detect markers related to the progression of normal ovarian epithelium to EOC and markers suitable for the classification of ovarian tumors. Three types of antigens will be examined: 1) blood group antigens, 2) ectopeptidases, and 3) mucins. Mucins will be studied in more detail using biochemical and molecular techniques in order to understand the role played by mucins in EOC. Also, novel methods for the analysis of the carbohydrate content of glycoproteins and mucins will be developed. The accomplishment of theses goals will provide new reagents for the diagnosis and therapy of EOC and will lead to an understanding of the antigenic changes associated with the malignant transformation of normal ovarian epithelium, tumor progression, and the biological behavior of ovarian tumors.