CORE--ANALYTICAL CORE

在一个多研究者、多项目的计划项目中， 需要科学和行政支助活动。 这些最好以集中的方式提供，而不是 试图根据一个特定的项目来分割它们 和/或调查员，特别是当有许多领域， 重叠 支持核心提供了这种集中的方法 并且基于两个组件。 分析部分将 为各种结构项目提供支持， 药物和代谢物的定性和定量分析。 气体 将进行色谱/质谱分析 通过使用稳定同位素，具有高灵敏度和特异性 稀释法结合电子俘获 负化学电离 液相色谱/电喷雾 电离串联质谱法将用于 药物代谢物的鉴定。 替代质谱法 采用电子电离、正化学 电离，液体二次离子，基质辅助激光解吸， 如果需要的话。 该部分还将提供 用于合成药物代谢物和稳定同位素的资源 类似物用作稳定同位素稀释测定的标准。 需要常规HPLC的分析方法 如果需要，将在核心中制定方法， 个别项目。 行政部门将提供 支持基本但与科学无关的 对个别项目的活动。 例如，行政 协调、财务管理、订购供应品、手稿 和赠款报告的编写，以及提供共同 复印、服务合同、邮寄和旅行等服务 支持等。此外，生物统计支持的所有 项目将由核心提供。

In a multi-investigator, multi-project Program Project, a number of scientific and administrative support activities are required. These are best provided in a centralized fashion rather than attempting to fragment them according to a particular project and/or investigator, especially when there are many areas of overlap. The Support Core provides such centralized approaches and is based on two components. The analytical component will provide support to the various Projects for the structural, qualitative and quantitative analysis of drugs and metabolites. Gas chromatography/mass spectrometry analyses will be performed with high sensitivity an specificity by the use of stable isotope dilution methodology in combination with electron capture negative chemical ionization. Liquid chromatography/electrospray ionization tandem mass spectrometry will be employed for the identification of drug metabolites. Alternative mass spectrometry methodology that employs electron ionization, positive chemical ionization, liquid secondary ion, matrix-assisted laser desorption is available should it be required. This component will also provide a resource for the synthesis of drug metabolites and stable isotope analogs for use as standards for stable isotope dilution assays. Analytical procedures that require conventional HPLC methodology will be developed in the Core if they are required in the individual projects. An administrative component will provide the structure to support essential but non-scientifically related activities for the individual Projects. For example, administrative coordination, fiscal management, ordering of supplies, manuscript and grant-report preparation, and the provision of common services such as duplicating, services contracts, mailing and travel support, etc. In addition, biostatistical support for all of the Projects will be provided by the Core.