CORE--AMBIENT PARTICLE EXPOSURE AND CHARACTERIZATION FACILITY

该核心将负责生成和 用于进行吸入的环境气溶胶的特性 曝光。我们拥有的环境粒子浓缩器系统 最近开发的将用于产生环境气溶胶在 在受控温度和相对温度下的理想水平 湿度条件。 使用多个连续的和时间集成的采样器 颗粒的物理、化学和生物特性 供应给吸入室的物质将被确定。粒子 质量浓度、粒度分布、无机离子和强离子 酸度、微量金属、元素碳以及真菌和花粉 将测定孢子和内毒素浓度。在……里面 此外，颗粒的氧化生成电位将是 量过了。此外，还将收集颗粒样本，用于 随后的体外细胞研究。最后，气态空气污染物 浓度，包括一氧化碳、氨、氮 二氧化氮和一氧化氮将受到监测。取决于可选的 实验条件、臭氧、硝酸、亚硝酸和硫磺 二氧化碳浓度也将被测量，当扩散时 不在曝光室的上游放置导流管系统以 把这些气体移走。 此核心与其他项目相关，因为它提供 In和In中环境气溶胶的产生和表征 体内和体外研究。此外，由于核心包括 大气科学和生物统计学的专家，它将提供 支持所有项目了解其来源和类型 并将观察到的生物效应与 各种测量的粒子属性。因此，这样做的目的是 核心不仅仅是提供工程专业知识和设施 用于产生和表征曝光气氛，但是 也将暴露和健康影响的研究整合到这个 项目。这对这两个国家都至关重要 对收集到的数据进行解释和综合。

This Core will be responsible for the generation and characterization of the ambient aerosols to conduct inhalation exposures. The ambient particle concentrator system that we have recently developed will be used to generate ambient aerosols at desirable levels, under controlled temperature and relative humidity conditions. Using a number of continuous and time-integrated samplers the physical chemical, and biological properties of the particles supplied to the inhalation chamber will be determined. Particle mass concentration, size distribution, inorganic ions and strong acidity, trace metals, elemental carbon, as well fungal and pollen spores and endotoxin concentrations will be determined. In addition, the oxidation generating potential of particles will be measured. Furthermore, particle samples will be collected for subsequent in vitro cellular studies. Finally, gaseous air pollutant concentrations, including carbon monoxide, ammonia, nitrogen dioxide, and nitric oxide will be monitored. Depending on optional experimental conditions, ozone, nitric and nitrous acids, and sulfur dioxide concentrations will also be measured, when a diffusion denuder system is not placed upstream of the exposure chamber to remove these gases. This Core relates to the other Projects because it proviede generation and characterization of ambient aerosols for both in vivo and in vitro studies. In addition, since the core includes experts in atmopheric sciences and biostatistics, it will provide support to all projects in understanding the sources and types of particles and in relating the observed biological effects to the various measured particle attributes. Therefore, the intent of this core is not only to provide the engineering expertise and facilities for generating and characterizing the exposure atmospheres but also to integrate the exposure and health effects studies in this project. This will be of paramount importance for both the interpretation and synthesis of the collected data.