CORE--MR SPECTROSCOPY

核磁共振光谱分析核心(C)的总体目标是开发和 应用多核核磁共振(核磁共振)光谱和 用于研究缺氧缺血性脑损伤的影像核心设备 在未成熟的实验动物身上。地核的光谱成分 将开发核磁共振波谱技术来评估代谢 在脑缺氧缺血期间和之后发生的变化 在未成熟的老鼠和新生的狗身上。空间定域31P和1H核磁共振 光谱学将被用来量化乳酸的变化。 浓度、能量代谢、细胞内pH和游离胞浆 镁浓度。我们开发了一种新的迭代方法来 更准确地测定细胞内pH值和游离镁(镁++) 浓度利用三磷酸腺苷的3个共振。 岩芯的成像部件将发展和应用磁力 磁共振成像(MRI)技术评价围产儿缺氧缺血 脑部受损。这项研究的核心将是对 正常发育的大鼠大脑。将使用两种成像技术来 阐明不同状态下脑损伤的部位和严重程度 缺氧--缺血和恢复。这些技术包括扩散和 T2加权自旋回波成像。初步结果表明， 弥散成像是早期发现低氧血症的敏感方法 缺血性损伤，而T2加权自旋回波成像更准确 监控长期损害。 动物移动限制装置是获得可靠的 漫射图像将不断地设计、构建和完善。 这些设备将被合并到通用探头设计中 每个研究项目的光谱学或成像(项目1、3、4)

The overall objective of the MR Spectrometry Core (C) is to develop and apply multinuclear Nuclear Magnetic Resonance (NMR) spectrometry and imaging core facilities for the study of hypoxic-ischemic brain damage in immature experimental animals. The spectroscopy component of the Core will develop NMR spectroscopy techniques to evaluate metabolic alterations which occur during and following cerebral hypoxia-ischemia in immature rats and newborn dogs. Spatially localized 31P and 1H NMR spectroscopy will be used to quantitate changes in lactate concentrations, energy metabolism, intracellular pH and free cytosolic magnesium concentrations. We have developed a new iterative method to determine more accurately intracellular pH and free magnesium (Mg++) concentrations using the 3 resonances of ATP. The imaging component of the Core will develop and apply magnetic resonance imaging (MRI) techniques to evaluate perinatal hypoxic-ischemic brain damage. Central to the research will be the characterization of the normal developing rat brain. Two imaging techniques will be used to elucidate the sites and severities of brain damage at different states of hypoxia-ischemia and recovery. These techniques include diffusion and T2-weighted spin echo imaging. Preliminary results indicate that diffusion imaging is a sensitive method for early detection of hypoxic- ischemic injury, while T2-weighted spin echo imaging more accurately monitors long-term damage. Animal movement restraining devices essential to obtaining reliable diffusion images will be continually designed, constructed and refined. These devices will be incorporated into the general probe design for spectroscopy or imaging for each research project (Projects 1,3,4)