CYTOSKELETON MEMBRANE INTERACTIONS AT CELL SUBSTRATE ADHESIONS

研究骨形成对于了解骨质疏松症具有重要价值。 骨质疏松症的发病机制与改善骨科保留力 植入物。我们正在研究移动，焦点接触和 破骨细胞的足体。细胞骨架-膜相互作用 细胞-底物粘连将使用FESEM的组合进行分析 和能量过滤瞬变电磁技术。我们将在研究中包括 局灶性接触/粘连斑块 成纤维细胞和神经生长锥体。此外，我们还将研究 细胞-底物粘连(足体)的花环状结构 在成骨过程中发现于破骨细胞薄层 再吸收。我们的观察将包括：i)FESEM成像 用LIM和RIS方法测量完整和三角剖分的腹面 (1993)，包括适当蛋白质的免疫金标记法。2) Triton松解术后的背侧切面相同。3)厚板的FESEM成像 提取后与底物成直角的细胞切片 EPON(Ris和Malecki，1993)。4)细胞粘连的成像 整体安装和厚切片的能量过滤透射电子显微镜。

Studying bone formation is of great value for understanding the mechanisms of osteoporosis and improving the retention of orthopedic implants. We are studying the locomotion, focal contacts and podosomes of osteoclasts. Cytoskeleton-membrane interactions at cell-substrate adhesions will be analyzed using a combination of FESEM and Energy Filtering TEM techniques. We will include in our study focal contacts/adhesion plaques characteristically found in fibroblasts and nerve growth cones. In addition, we will also study the rosette-like structure of cell-substrate adhesions (podosomes) found in the thin peripheral lamellae of osteoclasts during bone resorption. Our observations will include: I)FESEM imaging of the intact and Triton-lysed ventral surface by the method of Lim and Ris (1993), including immunogold labeling of the appropriate proteins. 2) same of the dorsal view after Triton lysis. 3) FESEM imaging of thick sections of cells at right angle to the substrate after extraction of the epon (Ris and Malecki, 1993). 4) Imaging of cell adhesions in whole mounts and thick sections by Energy Filtering TEM.