EXPERIMENTAL TRANSMISSION OF HTLV I TO NON HUMAN PRIMATE: SIV

这个合作项目已经建立了一个动物模型 原发HTLV-I的非人灵长类动物中的HTLV-I相关性疾病 病毒分离株，来自一名与HTLV-I相关的人类患者 热带痉挛性瘫痪(TSP)。三只接种恒河猴中的两只 猕猴被感染，并显示出疾病的迹象。一只动物 出现了与原始患者相同的发现， 有肌肉萎缩、多发性肌炎和关节炎。第二种动物 双重感染了SIV并出现了暗示 阴燃HTLV-I在SIV存在下诱发白血病 免疫缺陷疾病。这部作品出版了。另外五个 动物被接种了同样的HTLV-I型分离物。五次中的四次 在外周循环中检测到持续的病毒抗原 与弱的体液反应有关，而第五种动物是 持续的抗原阴性与强烈的体液有关 回应。这些结果表明，免疫反应很重要。 在猕猴模型中控制HTLV-I病毒的表达。这 这项工作是在一次全国会议上提出的， 出版了。我们目前正在开发自体 来自每种动物的转化细胞系以及 痘苗病毒-HTLV-I病毒构建以表征

This collaborative project has established an animal model of HTLV-I associated disease in non-human primates with a primary HTLV-I viral isolate, derived from a human patient with HTLV-I associated tropical spastic paraparesis (TSP). Two of three inoculated rhesus macaques were infected and showed signs of disease. One animal presented with a findings identical to that in the original patient, with muscle atrophy, polymyositis, and arthritis . The second animal was dually infected with SIV and developed signs suggestive of smoldering HTLV-I induced leukemia in the presence of SIV immunodeficiency disease. This work is published. Five additional animals were inoculated with this same HTLV-I isolate. Four of five had persistent viral antigen detection in the peripheral circulation associated with weak humoral responses, while the fifth animal was persistently antigen negative associated with a strong humoral response. These results suggest that immune responses are important in controlling HTLV-I virus expression in the macaque model. This work was presented at a national conference and the abstract published. We are currently in the process of developing autologous transformed cell lines from each of the animals as well as and vaccinia-HTLV-I virus constructs to characterize the