DEVELOPMENT OF DNA VACCINES FOR CONTRACEPTION

这是一项试验性研究， 雄激素依赖性附睾特异性蛋白 与精子功能有关。 理由是， 对雌性来说，随着抗体的分泌进入雌性生殖系统 道，会干扰受精。 与之相关的DNA 将EP 2的表达用作抗原，皮内施用 使用基因枪 在8只被注射的雌性中， 抗体形成的证据。 虽然可能延迟，但妊娠 没有被抑制。 今后的工作将致力于优化 疫苗接种时间表和DNA途径的改变 施用以在施用前最大化抗体产生。 生殖挑战

This is a pilot study to attempt to immunize male and female macaques to an androgen dependent, epididymis specific protein associated with sperm function. The rationale is that immunization of the female, with secretion of antibody into the female reproductive tract, will interfere with fertilization. The DNA associated with expression of EP2 was used as antigen, administered intradermally using a gene gun. Of eight females injected, two demonstrated evidence of antibody formation. Although possible delayed, pregnancy was not inhibited. Future work will be directed to optimization of the vaccination schedule and alteration of the route of DNA administration in order to maximize antibody production prior to reproductive challenge.