PURIFICATION OF NOVEL FACTOR INVOLVED IN PRODUCTION OF APO B48

现已证实，增加血浆胆固醇 这一水平与心脏病风险的增加有关。这个 血液中胆固醇的主要载体是一种名为 载脂蛋白B因为载脂蛋白B具有如此重要的生理作用，我们 研究这种蛋白质是如何产生的。载脂蛋白B有两种形式 在血浆中，大的形式apo-B100和小的形式apo-B48， 大约是apo-B100的一半大小。载脂蛋白-B48在 饮食中胆固醇的吸收和运输。我们发现， 有一种不寻常的机制导致了这一现象的产生 Apo-B蛋白的简称。我们一直在调查这件事 在生化和分子水平上的作用机制。我们发现， 参与这一途径的一个因子在高表达 在狒狒肾脏中的含量。因此，我们正在努力净化这一点 为了鉴定和了解其主要成分，我们对其进行了研究。 在载脂蛋白B48合成中的作用。我们的研究结果将 洞察身体如何处理饮食中的胆固醇。我们的 长期目标是利用这些信息来设计可能 有助于降低血液中的胆固醇水平，预防糖尿病的发生 动脉硬化。

It is now well established that increased plasma cholesterol levels are associated with an increased risk of heart disease. The main carrier of cholesterol in the blood stream is a protein called apo-B. Because apo-B has such an important physiological role, we are studying how this protein is produced. There are two forms of apo-B in the plasma a large form, apo-B100, and a small form, apo-B48, which is about half the size of apo-B100. Apo-B48 is important in the absorption and transport of dietary cholesterol. We have found that there is an unusual mechanism responsible for the production of this short form of the apo-B protein. We have been investigating this mechanism at the biochemical and molecular level. We have found that one of the factors involved in this pathway is expressed at high levels in baboon kidney. Therefore, we are trying to purify this factor from baboon kidney in order to identify it and understand its role in the synthesis of apo-B48. The results from our research will provide insight into how the body handles dietary cholesterol. Our long-term goal is to use this information to design therapies that may help lower blood cholesterol levels and prevent the development of atherosclerosis.