TRITIATION OF MANDUCA ADIPOKINETIC HORMONE

Manduca脂肪运动激素类似物的生物测定和 在受体结合分析中，单一氨基酸替换类似物 Manduca脂肪运动激素(M-AKH) PGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-GlyNH2的活性 生物分析以及受体结合分析。氨基酸是 被丙氨酸和D-类似物取代。此外，扩展的M-AKH和 测试了含有光亲和标记的类似物。所有的类比都有 活动减少。所有具有足够活性的多肽 完整的剂量反应曲线达到与天然的相同的最大活性。 M-AKH。使用类比，其中L-Phe4由Ala或由Ala代替 D-Phe和L-Thr3被D-Thr取代，竞争对手带来了改进 在我们的竞争性受体结合试验中M-AKH的结合。在 生物活性测定表明，灭活浓度的Ala4 M-AKH可增加 半最适浓度的天然M-AKH的活性。多肽， 19,481-486(1998)。

Analogs of Manduca Adipokinetic-Hormone Tested in a Bioassay and in a Receptor-Binding Assay Single amino acid replacement analogs of Manduca adipokinetic hormone (M-AKH) pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-GlyNH2 were tested for activity in bioassays as well as receptor binding assays. Amino acids were replaced by Ala and by D-analogs. In addition an extended M-AKH and analogs containing photo affinity labels were tested. All analogs had reduced activity. All the peptides which had enough activity to allow a full dose response curve reached the same maximal activity as native M-AKH. The use of analogs, in which L-Phe4 was replaced by Ala or by D-Phe and L-Thr3 replaced by D-Thr, as competitors led to improved binding of M-AKH in our competitive receptor binding assays. In the bioassay an inactive concentration of Ala4 M-AKH increased the activity of a half optimal concentration of native M-AKH. Peptides, 19, 481-486 (1998).