ROLE OF MOTOR PROTEINS IN FUNGI MITOSIS

（部分由NSF MCB 9408249向J. Aist提供支持）。 这个项目 目前正在进行中。 它的目标是确定 在分裂后期B， 真菌赤壳菌，并探讨作用， 细胞质动力蛋白在有丝分裂、核迁移和核分裂中的作用 定位 为了实现前两个目标， 用于在控制细胞的后期切断纺锤体， 胞质动力蛋白突变体。 为了实现后两个目标， 光学陷阱用于测量阻止所需的相对力 核迁移和取代细胞核。 到 数据显示纺锤体两极会自己拉开 这一过程是由细胞质介导的， 动力蛋白 部分工作已摘录至1997年ASCB 目前正在编写两份文件供出版。 Inoue，S.，O.C. Yoder，B.G. Turgeon和J.R.艾斯特 04 The Fantasy（1997） 动力蛋白对真菌中紫苑的形成和功能至关重要 红球藻 Molec. 生物细胞8：

(Supported in part by NSF MCB 9408249 to J. Aist). This project is currently in progress. Its goals are to determine where the forces are generated to separate the spindle poles during anaphase B in the fungus Nectria haematococca, and to investigate the role of cytoplasmic dynein in mitosis, nuclear migration and nuclear positioning. To achieve the first two goals the laser microbeam is used to sever spindles during anaphase in control cells and in cytoplasmic-dynein mutants. To achieve the latter two goals the optical trap is used to measure the relative forces needed to arrest nuclear migration, and displace nuclei, in controls and mutants. To date the data reveal that the spindle poles pull themselves apart during anaphase and that this process is mediated by cytoplasmic dynein. Parts of this work have been abstracted to the 1997 ASCB meeting and two papers are currently being prepared for publication. Inoue, S., O.C. Yoder, B.G. Turgeon and J.R. Aist. (1997) Cytoplasmic dynein is essential for formation and function of asters in the fungus Nectria haematococca. Molec. Biol. Cell 8: