ROLE OF ARACHIDONIC ACID IN HUMAN MONOCYTE PHAGOCYTOSIS

(部分由NIH GM 45983和AHA93-3030G至M支持。 Lennartz)。我们已经证明花生四烯酸(AA)是 吞噬作用所必需的，而且显然是独一无二的 (吞噬)磷脂酶(PPL)负责AA的释放。 抑制PPL导致电子发光体积聚 人单核细胞胞浆中的小泡(ELV)。ELV不会 用阳离子铁蛋白标记，表明它们不是连续的 与质膜结合。我们的目标是用HVEM做得更好 在3-D中可视化ELV的形态，并对数量进行量化 含有免疫金标记物的囊泡 与牛血清白蛋白孵育。记录了许多立体声、HVEM和IVEM图像 从0.5到1.0(m厚的截面)。数据清楚地表明， 问题中的囊泡与质膜不连续， 看起来不像是以网络排列方式连接的。伦纳尔茨 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 史密斯。(1997)对磷脂酶A2的抑制导致对 免疫球蛋白介导的质膜进入电子透亮囊泡 吞噬作用。《细胞科学》，110：2041-2052。传播和 培训子项目

(Supported in part by NIH GM 45983 and AHA 93-3030G to M. Lennartz). We have demonstrated that arachidonic acid (AA) is required for phagocytosis to occur and that an apparently unique (phagocytic ) phospholipase (pPL) is responsible for AA release. Inhibition of pPl results in the accumulaiton of electron lucent vesicles (ELV) in the cytosol of human monocytes. The ELV do not label with cationized ferritin, suggesting they are not contiguous with the plasma membrane. Our goal is to use the HVEM to better visualize the morphology of the ELV in 3-D, and to quantify the number of vesicles that contain an immunogold label when the cells are incubated with BSA. Many stereo HVEM and IVEM images were recorded from 0.5 and 1.0 (m thick sections. The data clearly reveal that vesicles in questions are no continuous with the plasma membrane and do not appear to be connected in a network arrangement. Lennartz, M.R., A.F.C. Yuen, S. M. Masi, D.G Russell, K.F. Buttle and J.J. Smith. (1997) Phospholipase A2 inhibition results in sequestration of plasma membrane into electron lucent vesicles during IgG-mediated phagocytosis. J. Cell Sci., 110:2041-2052. DISSEMINATION AND TRAINING SUBPROJECTS