FATE OF VERTEBRATE CELLS LACKING CENTROSOMES

（部分由NIH GMS 40198支持，C.L.里德尔）。 概述的 在我们的一个TRD项目中，我们开发了一些方法， 通过使用GFP标记的γ微管蛋白产生无中心体细胞， 点燃中心体，然后用激光破坏 显微手术 使用这种方法，我们正在研究的作用 CV1（绿猴肾）细胞周期的中心体 成纤维细胞）细胞。 我们要问的第一个问题是 现有的微管阵列被修改时，中心体， 破坏了中心体并分解后， 现有的微管通过冷却，它的改革时，细胞是 重新加热。 我们还想知道缺乏中心体的细胞是否 能够在细胞周期中正常进行。 唯一的办法 进行这些研究的方法是使用BMIRR激光设备， 无中心体细胞。

(Supported in part by NIH GMS 40198 to C.L. Rieder). As outlined in one of our TRD projects, we have developed methods that allow us to generate centrosome free cells by using GFP-tagged gamma tubulin to light up the centrosome for subsequent destruction by laser microsurgery. Using this approach we are studying the role of the centrosome during the cell cycle in CV1 (greeen monkey kidney fibroblast) cells. The first question we are asking is how the existing microtubule array is modified when the centrosome is destroyed and if, after destroying the centrosome and disassembling the existing microtubules by cooling, it reforms when the cell is re-warmed. We are also asking if cells lacking centrosomes are capable of proceeding normally through the cell cycle. The only way to conduct these studies is to use the BMIRR laser facility to create centrosome-free cells.