CRYSTAL STRUCTURE OF HUMAN APOLIPOPROTEIN A I

从冷冻保护的(30%葡萄糖)中收集衍射数据 载脂蛋白A-I载脂蛋白((1.43)A-I晶体)(P212121，a=97.47，b= 113.87，c=196.19)1997年7月13日在国际象棋光束线A-1(100K，70 0.5‘振荡框，60秒，探测器距离130 mm)。数据 与MOSFLM集成、使用Scala进行扩展并进行进一步处理 CCP4套间。这些数据在3.0分辨率下完成了57%， Rsym为11.4%(3.2 3.0外壳为37.9%)。这些数据是 比我们在实验室里收集的数据要好得多 用于在4个分辨率下求解结构。然而，即使在100K， 这些晶体对辐射损伤非常敏感， 衍射率仅为3，持续30分钟。我们使用国际象棋光束线A-1 这个项目验证了这些apo((143)A-I)的概念 晶体会产生衍射光，从而提高分辨率。其他数据 国际象棋计划收集这些数据以完成这些数据，以便 在3点可以开始精炼。我们也期待着未来的数据 从我们的低盐I222 apo((143)A-I晶体)中收集国际象棋 形式，以及我们正在研究的其他形式的载脂蛋白A-I的晶体 实验室。

Diffraction data were collected from a cryoprotected (30% glucose) apolipoprotein A-I apo ((1 43)A-I crystal (P212121, a = 97.47, b = 113.87, c = 196.19 ) at CHESS beamline A-1 on 13 July 1997 (100 K, 70 0.5' oscillation frames, 60 sec, 130 mm detector distance). The data were integrated with MOSFLM, scaled with SCALA, and further processed with the CCP4 suite. These data are 57% complete to 3.0  resolution, with an Rsym of 11.4% (37.9% in the 3.2 3.0  shell). These data are dramatically better than those that we collected in our laboratory and used to solve the structure at 4  resolution. Even at 100 K, however, these crystals remain extremely sensitive to radiation damage, diffracting to 3  for only 30 min. Our use of CHESS beamline A-1 on this project has validated the notion that these apo ((1 43)A-I crystals diffract to better that 4  resolution. Additional data collection at CHESS is planned to complete these data, so that refinement at 3  can commence. We also look forward to future data collection at CHESS from our low-salt I222 apo ((1 43)A-I crystal form, and crystals of other forms of apo A-I under study in our laboratories.