SINGLE PHOTON COUNTING FLUORESCENCE & FIELD SPECTROSCOPY & IMAGE APPARATUS

时间相关单光子计数(TCSPC)荧光 仪器继续作为最先进的光谱分析仪器运行 乐器。与开发新技术的目标一致 在现有仪器之外，一种时间分辨的近场光学 光谱仪(TNOS)，于今年在RLBL首次开发， 彻底改变了分子动力学和光子过程的研究 通过融合近场的非凡空间分辨率 最先进的超快野外光学探测技术 光谱方法。我们的仪器设计用于采集单条信号 使用三种对比度机制定位或扫描样本图像 (力、强度和激发态寿命)，四，快 转换，光学配置。现代时间域谱 分辨率，由时间相关信号的应用确定 光子计数法，是20皮秒。视样品而定 值得注意的是，RLBL-TNO可以配置在反射中或 传输几何形状；对于每个光学几何形状，近场 光纤探头可以在照明或采集中实现 模式。对比机构与仪器设计的多样性 据我们所知，多功能性是现有近场无与伦比的 光学技术。其他发展包括将 来自跨国公司的传统TCSPC系统。检测中的这种分离 和电子产品，这将促进两者的最大限度利用 仪器，已经接近完成。单独的计算机数据采集 TCSPC中心即将完工。频率计数 方法也被纳入到这个系统中。

The time-correlated single photon counting (TCSPC) fluorescence apparatus continues to perform as a state-of-the art spectroscopic instrument. In accord with the goal of developing new technologies out of existing instrumentation, a time resolved near-field optical spectrometer (TNOS), first developed at the RLBL within this year, revolutionizes the study of molecular dynamic and photonic processes by incorporating the extraordinary spatial resolution of the near field optical probe technique with state-of-the-art ultrafast spectroscopic methods. Our instrument is designed to acquire single location or scanned images of samples using three contrast mechanisms (force, intensity and excited state lifetime) in four, quickly converting, optical configurations. Current time domain spectroscopic resolution, determined by the application of time correlated single photon counting methods, is 20 picoseconds. Depending on the sample of interest, the RLBL-TNOS can be configured in the reflection or transmission geometry; and with each optical geometry, the near-field optical fiber probe can be implemented in illumination or collection mode. The multiplicity in contrast mechanisms and instrumental design versatility is to our knowledge unparalleled with existing near-field optical technology. Other developments include the separation of the conventional TCSPC system from the TNOS. This separation in detection and electronics, which will facilitate maximum use of both instruments, is nearly complete. A separate computer data acquisition center for the TCSPC is nearing completion. Frequency counting methods are also being incorporated into this system.