FAR FIELD MICROSCOPIC DETECTION

除了正在开发的其他显微技术外 对于时间相关单光子计数共焦显微镜， 正在为这种时间分辨光谱学而开发。 这些技术 将用于观察单个生物体的动态变化， 组件，例如核糖体，光收集器（见亮点）， 血红素蛋白、DNA寡聚体等。激光扫描共聚焦显微镜 提供低背景三维荧光图像， 远场时间分辨单分子检测。 我们有 结合倒置激光扫描共聚焦荧光显微镜 利用现有的皮秒染料激光器和时间相关的单脉冲激光器， 光子计数（TCSPC）检测电子系统。 检测 是基于低背景光子计数硅雪崩光电二极管 (APD)，其具有高的检测量子效率（在630 ° C下为60- 70 nm）此资源已被等待 设备可以被访问者使用。

In addition to the other microscopic techniques being developed for time-correlated single photon counting confocal microscopy, is being developed for such time-resolved spectroscopy. These techniques will be used to observe dynamical changes in single biological assemblies, e.g. ribosomes, light harvesters (see Highlights), hemoproteins, DNA oligomers, etc. Laser scanning confocal microscopy provides low background three dimensional fluorescence images and time-resolved single molecule detection in the far field. We have combined an inverted laser scanning confocal fluorescence microscope with the existing picosecond dye laser and the time-correlated single photon counting (TCSPC) detection electronic systems. The detection is based on a low background photon counting Si avalanche photodiode (APD), which has a high detection quantum efficiency (60-70% at 630 nm) This Resource is already inundated with users waiting for the apparatus to become useable by visitors.