IN VIVO IN UTERO MICROSCOPIC MRI OF RAT FETUS W/ CONGENITAL DIAPHRAGMATIC HERNIA

子宫解剖翻转术中的应用 畸形是在小型哺乳动物模型中发展起来的。一种新的设计 新技术缺乏非侵入性的预和 手术后胎儿监护。我们已经应用了高分辨率核磁共振 以活体、宫内显像法建立大鼠先天性心脏病模型 横隔疝(CDH)。先天性心脏病是一种发育异常， 包括横隔膜的不完全关闭，肝脏的突出 和腹部脏器进入胸腔，以及肺发育不全。 在怀孕19-22天(每天一次)对8只水坝进行成像 诊断CDH的存在或不存在，并监测 做手术。在第19天被显示为CDH+的患者接受了 即刻外科气管结扎术逆转肺发育不良 并迫使腹部内容物从胸腔排出。39只大鼠 胎儿使用多层T2加权快速自旋回波成像 序列在4T全身成像系统(GE，Signa)上。病理学和 手术结果是通过高分辨率尸检确认的 成像(9.4T)和随后的显微镜解剖。这 信息将有助于宫内发育的使用和发展 先天畸形的介入治疗。

In Utero surgical techniques for the reversal of anatomical malformations are developed in small mammalian models. The design of new techniques suffers from lack of non-invasive pre- and post-surgical fetal monitoring. We have applied high resolution MRI to in-vivo, in-utero imaging of a rat model for Congenital Diaphragmatic Hernia (CDH). CDH is a developmental anomaly which involves incomplete closure of the diaphragm, herniation of the liver and abdominal viscera into the thoracic cavity, and lung hypoplasia. Eight dams were imaged on days 19-22 of gestation (once a day) to diagnose the presence or absence of CDH and monitor the effects of surgery. Those who were shown to be CDH+ on day 19 underwent immediate surgical tracheal ligation to reverse pulmonary hypoplasia and force the abdominal contents from the thoracic cavity. 39 rat fetuses were imaged using a multislice, T2 weighted, fast spin echo sequence on a 4T whole body imaging system (GE, Signa). Pathology and results of surgery were confirmed post-mortem by high resolution imaging (9.4T) and subsequent microscopic dissection. This information will help in the use and development of in utero intervention for treatment of congenital abnormalities.