ASSEMBLY OF CORNIFIED CELL ENVELOPE IN TERMINALLY DIFFERENTIATED KERATINOCYTES

哺乳动物表皮角质形成细胞的终末分化 最终形成一个角蛋白细丝基质，包裹在一张 称为角化细胞囊膜的共价交联蛋白质 (Ce)。CE的生物发生在正常和 疾病状态，如牛皮癣。-STEM测量质量/面积 纯化的CES使人们能够评估它们的同质性并测试 在一个给定的CE中可能的专业领域。从人中分离的CES 表皮(正常和银屑病)，小鼠表皮(正常和 转基因)、其他上皮细胞和培养的角质形成细胞正在 学习。研究了几种不同的制备方法。 因为CE和碳衬底之间的残留洗涤剂可以 影响质量测量。的平均厚度和质量/面积 无论组织是什么，成熟的包膜都惊人地相同。 这些结果有助于定义当前的CE组装模型。

Terminal differentiation of mammalian epidermal keratinocytes eventually creates a keratin filament matrix encased within a sheet of covalently cross-linked proteins called the cornified cell envelope (CE). Biogenesis of the CE is being studied in both normal and diseased states, e.g. psoriasis. - STEM measurements of mass/area on purified CEs allow one to evaluate their homogeneity and test for possible specialized areas within a given CE. CEs isolated from human epidermis (normal and psoriatic), mouse epidermis (normal and transgenic), other epithelia, and cultured keratinocytes are being studied. Several different methods of preparation have been examined since residual detergent between the CE's and the carbon substrate can affect mass measurements. The average thickness and mass/area of the mature envelopes are amazingly the same regardless of the tissue. Tliese results are helping to define the current model of CE assembly.