LABELING OF ADENOVIRUS 12 KNOB

腺病毒蛋白A12参与了与 病毒传播到受体。用6-组氨酸克隆了该蛋白 尾巴用于纯化，凝血酶部位用于去除His-Tail。一个 合成了NiNTA-纳米金探针，并用该蛋白对其进行了检测， 这是kDa的三聚体(一个很好的STEM样本)。这个 以凝血酶裂解蛋白作为对照。几乎每一种蛋白质 他的尾巴上贴着标签，通常是2-3个金色的簇， 与它是一个三聚体相一致。通过STEM，非His蛋白 (裂解)显示没有簇结合。

The adenovirus protein, A12, is involved in the binding of the virus to the receptor. The protein has been cloned with 6-histidine tail for purification and a thrombin site to remove the his-tail. A NiNTA-Nanogold probe was synthesized and tested with this protein, which is a trimer of 64 kDa (a good STEM sample). The thrombin-cleaved protein was used as a control. Nearly every protein with the his-tail was labeled, often with 2-3 gold clusters, consistent with it being a trimer. By STEM, the non-his protein (cleaved) showed no cluster binding.