DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

美国国立卫生研究院动物遗传资源（NIHAGR）保存了几个动物物种，以及一个物种内许多宝贵的基因型，这些基因型被用作生物医学研究的模型。为了保护这种种质，建立了胚胎库，作为一种经济的手段，无限期地保存这些啮齿动物和兔子的种群和品系。胚胎冷冻保存对基因型管理还有其他好处，如防止因疾病、生殖失败、自然灾害或遗传污染而导致的种质损失。cryobank通过提供一种资源来周期性地重新衍生已定义的基因型，以重新标准化基础育种群体，从而最大限度地减少遗传漂变造成的变化。作为公认的实验动物物种储存库，NIHAGR根据生物医学研究界的要求定期提供繁殖细胞核。与运送活体动物相比，冷冻胚胎是一种更简单、更人性化、更容易在全球范围内运输所需种质的方法。胚胎处理、冷冻保存和移植程序的另一个重要影响是消除一些小鼠疾病的垂直传播。基因型和物种差异继续影响这一努力的效率。-小鼠胚胎，冷冻保存，胚胎库，动物模型

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species, and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases. Genotype and species differences continue to impact on the efficiency of this is effort. - mouse embryos, cryopreservation, embryo banking, animal models