DEVELOPMENT OF EMBRYO CRYOPRESERVATION PROCEDURES FOR LABORATORY RATS

美国国立卫生研究院动物遗传资源（NIHAGR）保留了许多独特的大鼠基因型，用作人类正常和疾病过程的模型。胚胎长期冷冻保存程序的发展将促进对这一资源的有效管理。为了建立实验室大鼠的冷冻保存种质库，必须建立胚胎库和再分化过程的每个步骤的方案。这将包括胚胎供体雌性的准备和交配，胚胎收集，冷冻保存，解冻和胚胎移植。先前的研究表明，为实验室小鼠开发的超排卵程序在应用于实验室大鼠时并不有效。本项目的主要目的是评估使用促性腺激素释放激素（GnRH-a）类似物作为同步发情周期的替代方法，用于胚胎收集和冷冻保存。不同模型的胚胎收集效率的差异与模型的育种性能相似。然而，迄今为止，在开发所有大鼠模型的冷冻保存胚胎库方面取得了进展。-大鼠胚胎，冷冻保存，胚胎库，动物模型

The NIH Animal Genetic Resource (NIHAGR) maintains many unique rat genotypes used as models of normal and disease processes in man. Effective management of this resource would be facilitated by the development of embryo cryopreservation procedures for long-term banking. In order to develop banks of cryopreserved germ plasm from laboratory rats, protocols for each step of the embryo banking and rederivation process must be established. These would include the preparation and mating of embryo donor females, embryo collection, cryopreservation, thawing, and embryo transfer. Previous studies suggest that superovulation procedures developed for laboratory mice are not as effective when applied to laboratory rats. The principal objective of this project is to evaluate treatment with an analog of gonadotropin releasing hormone (GnRH-a) as an alternative approach to synchronize estrus cycles for embryo collection and cryopreservation. The efficiency of embryo collection varies between models in a manner similar to that of the breeding performance of the model. However, progress has been made in developing banks of cryopreserved embryos from all rat models examined to date. - rat embryo, cryopreservation, embryo bank, animal models