Phototoxicity using the Chorioallantoic Membrane

描述(申请人摘要)：此建议，“使用光毒性筛选 绒毛尿囊膜“(PhotoCAM)是一种替代光毒性 用于鉴定具有光毒性的化学介体和物质的筛选试验 属性，这是制药、化学品和消费者的需求 产品行业。能育鸡的绒毛尿囊膜(CAM) 鸡蛋已经被证明是检测刺激物的一种有用的、敏感的模型。 这项建议的目的是为了证明采用 CaM分析，以及PGE2和细胞因子产生的终点 构成组织的表皮细胞，作为识别潜力的测试系统 光毒素或光刺激物。只有两篇论文发表在 文献使用这种模型，因此这是一种相对新颖的应用 这个模型系统。使用完整的组织比使用完整的组织有几个优点 现有的基于细胞培养的光毒性筛选方法。其中包括 在皮肤中发现多种细胞类型，并且很容易接触到 与具有代谢功能的肝脏接触的循环系统 组织。此处描述的PhotoCAM检测将提供一种灵敏的测试 系统，同时(1)直接遵守动物福利法(AWA) 通过减少动物使用来解决3R问题；(2)增加歧视 与细胞培养方法相比，产生的数据的功率和质量；以及 (3)大幅降低使用动物的相关成本 光毒性筛选研究。

DESCRIPTION (Applicant's Abstract): This proposal, "Phototoxicity Screen using the Chorioallantoic Membrane" (PhotoCAM) is an alternative phototoxicity screening test to identify chemical mediators and substances with phototoxic properties, which is in demand by the pharmaceutical, chemical, and consumer product industries. The chorioallantoic membrane (CAM) of the fertile chicken egg has already shown to be a useful, sensitive model for detecting irritants. The purpose of this proposal is to demonstrate the feasibility of employing the CAM assay, as well as endpoints of PGE2 and cytokine production by the epidermal cells comprising the tissue, as test systems to identify potential phototoxins or photoirritants. There have only been two papers published in the literature using this model, thus this is a relatively novel application of this model system. The use of an intact tissue has several advantages over existing cell culture-based phototoxicity screening assays. These include the presence of multiple cell types found in the skin, and a readily accessible circulatory system that is in contact with metabolically functional hepatic tissue. The PhotoCAM Assay described herein will provide a sensitive test system while (1) complying with the Animal Welfare Act (AWA) by directly addressing the 3Rs, by reducing animal usage; (2) increasing the discriminating power and quality of data generated when compared to cell culture methods; and (3) greatly reducing the costs associated with the use of animals for phototoxicity screening studies.